PMID- 24260688
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20131122
LR  - 20220330
IS  - 1993-0771 (Print)
IS  - 1993-078X (Electronic)
IS  - 1993-0771 (Linking)
VI  - 7
IP  - 1
DP  - 2013
TI  - Comparative cytogenetics of two endangered leuciscine fish, Squalius aradensis 
      and S. torgalensis (Teleostei, Cyprinidae), from the Iberian Peninsula.
PG  - 33-42
LID - 10.3897/CompCytogen.v7i1.4672 [doi]
AB  - In this study, the description of the karyotypes of the endangered chubs Squalius 
      aradensis (Coelho, Bogutskaya, Rodrigues and Collares-Pereira, 1998) and Squalius 
      torgalensis (Coelho, Bogutskaya, Rodrigues and Collares-Pereira, 1998) is 
      presented by means of conventional (Giemsa-staining, Chromomycin A3 
      (CMA3)-fluorescence, Silver-impregnation (Ag-NORs)) and molecular (fluorescence 
      in situ hybridization (FISH) with 18S rDNA probe) protocols. These endemic 
      sister-species have an allopatric but adjacent distribution in the most 
      southwestern part of the Iberian Peninsula. Diploid chromosome number was 
      invariably 2n = 50 and karyotypes of both species were grossly similar, composed 
      of metacentric and submetacentric elements with a reduced number of acrocentric 
      pairs. Sequential staining using FISH with an 18S rDNA probe, CMA3 and Ag-NORs 
      treatments revealed consistent positive signals located at the end of the short 
      arms of a submetacentric chromosome pair, likely homologous in both species. 
      While providing useful cytogenetic comparative data against other members of the 
      genus Squalius Bonaparte, 1837, the work aimed to draw attention towards the 
      conservation of two narrow-range and highly confined fish species.
FAU - Nabais, Catarina
AU  - Nabais C
AD  - Universidade de Lisboa, Faculdade de Ciencias, Centro de Biologia Ambiental, 
      Campo Grande, Lisboa, Portugal.
FAU - Rampin, Massimiliano
AU  - Rampin M
FAU - Collares-Pereira, Maria Joao
AU  - Collares-Pereira MJ
LA  - eng
PT  - Journal Article
DEP - 20130318
PL  - Bulgaria
TA  - Comp Cytogenet
JT  - Comparative cytogenetics
JID - 101503807
PMC - PMC3833748
OTO - NOTNLM
OT  - Cytotaxonomy
OT  - FISH with rDNA
OT  - Leuciscinae
OT  - NOR-phenotype
OT  - chromomycin A3
OT  - silver staining
EDAT- 2013/11/22 06:00
MHDA- 2013/11/22 06:01
PMCR- 2013/03/18
CRDT- 2013/11/22 06:00
PHST- 2013/01/12 00:00 [received]
PHST- 2013/02/14 00:00 [accepted]
PHST- 2013/11/22 06:00 [entrez]
PHST- 2013/11/22 06:00 [pubmed]
PHST- 2013/11/22 06:01 [medline]
PHST- 2013/03/18 00:00 [pmc-release]
AID - 10.3897/CompCytogen.v7i1.4672 [doi]
PST - epublish
SO  - Comp Cytogenet. 2013 Mar 18;7(1):33-42. doi: 10.3897/CompCytogen.v7i1.4672. 
      eCollection 2013.