PMID- 24261084
OWN - NLM
STAT- MEDLINE
DCOM- 20131218
LR  - 20191112
IS  - 1469-0667 (Print)
IS  - 1469-0667 (Linking)
VI  - 19
IP  - 2
DP  - 2013
TI  - Human blood plasma proteome mapping for search of potential markers of the lung 
      squamous cell carcinoma.
PG  - 123-33
AB  - Blood plasma proteomes obtained from 77 lung squamous cell carcinoma (LSCC) 
      patients (Stages I-III) and 67 healthy controls (all males) were analyzed by 
      using the label-free liquid chromatography tandem mass spectrometry (LC-MS/MS) 
      method for the search of potential cancer biomarkers. All plasma samples were 
      depleted of 14 highly-abundant plasma proteins by immune-affinity column 
      chromatography before LC-MS/MS. We identified and quantified 809 differential 
      proteins with molecular weights from 6.4 kDa to 3900 kDa using a label-free 
      method. Three hundred and sixty four proteins were identified in all three 
      groups. Changes in levels of an expression of blood plasma proteins associated 
      with LSCC were discovered. Among them, 43 proteins were overexpressed and 39 
      proteins were down-regulated by more than two-fold between the plasmas of lung 
      cancer patients and healthy men. We focused our attention on proteins whose 
      expression levels increased from control to early stage and then to advanced 
      stage tumor. Each of the 43 unique overexpressed proteins was classified 
      according to its cellular localization, biological processes, molecular function 
      and classes. Many of these proteins are involved in biological pathways pertinent 
      to tumor progression and metastasis and some of these deregulated proteins may be 
      useful clinical markers.
FAU - Shevchenko, Valeriy E
AU  - Shevchenko VE
AD  - Federal State Budgetary Institution N. N. Btokhin Russian Cancer Research Center 
      Under the Russian Academy of Medical Science, Moscow, Russia. vshev@nm.ru
FAU - Kovalev, Sergey V
AU  - Kovalev SV
FAU - Arnotskaya, Natalia E
AU  - Arnotskaya NE
FAU - Zborovskaya, Irina B
AU  - Zborovskaya IB
FAU - Akhmedov, Bakhrom B
AU  - Akhmedov BB
FAU - Polotskii, Boris E
AU  - Polotskii BE
FAU - Kostin, Aleksey U
AU  - Kostin AU
FAU - Moukeria, Anush F
AU  - Moukeria AF
FAU - Zaridze, David G
AU  - Zaridze DG
FAU - Davidov, Mikhail I
AU  - Davidov MI
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Mass Spectrom (Chichester)
JT  - European journal of mass spectrometry (Chichester, England)
JID - 101124748
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Blood Proteins)
RN  - 0 (Protein Hydrolysates)
RN  - 0 (Proteome)
RN  - EC 3.4.21.4 (Trypsin)
SB  - IM
MH  - Aged
MH  - Biomarkers, Tumor/*chemistry/*genetics
MH  - Blood Proteins/*chemistry/*genetics
MH  - Carcinoma, Squamous Cell/*chemistry/*genetics
MH  - Chromatography, High Pressure Liquid
MH  - Databases, Protein
MH  - Down-Regulation
MH  - Humans
MH  - Lung Neoplasms/*chemistry/*genetics
MH  - Middle Aged
MH  - Molecular Weight
MH  - Neoplasm Metastasis
MH  - Neoplasm Staging
MH  - Protein Hydrolysates/chemistry
MH  - Proteome/*genetics
MH  - Tandem Mass Spectrometry
MH  - Trypsin/chemistry
EDAT- 2013/11/23 06:00
MHDA- 2013/12/19 06:00
CRDT- 2013/11/23 06:00
PHST- 2013/11/23 06:00 [entrez]
PHST- 2013/11/23 06:00 [pubmed]
PHST- 2013/12/19 06:00 [medline]
AID - 10.1255/ejms.1220 [doi]
PST - ppublish
SO  - Eur J Mass Spectrom (Chichester). 2013;19(2):123-33. doi: 10.1255/ejms.1220.