PMID- 24261750
OWN - NLM
STAT- MEDLINE
DCOM- 20140219
LR  - 20211021
IS  - 1471-2148 (Electronic)
IS  - 1471-2148 (Linking)
VI  - 13
DP  - 2013 Nov 21
TI  - Reconstruction of the ancestral marsupial karyotype from comparative gene maps.
PG  - 258
LID - 10.1186/1471-2148-13-258 [doi]
AB  - BACKGROUND: The increasing number of assembled mammalian genomes makes it 
      possible to compare genome organisation across mammalian lineages and reconstruct 
      chromosomes of the ancestral marsupial and therian (marsupial and eutherian) 
      mammals. However, the reconstruction of ancestral genomes requires genome 
      assemblies to be anchored to chromosomes. The recently sequenced tammar wallaby 
      (Macropus eugenii) genome was assembled into over 300,000 contigs. We previously 
      devised an efficient strategy for mapping large evolutionarily conserved blocks 
      in non-model mammals, and applied this to determine the arrangement of conserved 
      blocks on all wallaby chromosomes, thereby permitting comparative maps to be 
      constructed and resolve the long debated issue between a 2n = 14 and 2n = 22 
      ancestral marsupial karyotype. RESULTS: We identified large blocks of genes 
      conserved between human and opossum, and mapped genes corresponding to the ends 
      of these blocks by fluorescence in situ hybridization (FISH). A total of 242 
      genes was assigned to wallaby chromosomes in the present study, bringing the 
      total number of genes mapped to 554 and making it the most densely 
      cytogenetically mapped marsupial genome. We used these gene assignments to 
      construct comparative maps between wallaby and opossum, which uncovered many 
      intrachromosomal rearrangements, particularly for genes found on wallaby 
      chromosomes X and 3. Expanding comparisons to include chicken and human permitted 
      the putative ancestral marsupial (2n = 14) and therian mammal (2n = 19) 
      karyotypes to be reconstructed. CONCLUSIONS: Our physical mapping data for the 
      tammar wallaby has uncovered the events shaping marsupial genomes and enabled us 
      to predict the ancestral marsupial karyotype, supporting a 2n = 14 ancestor. 
      Futhermore, our predicted therian ancestral karyotype has helped to understand 
      the evolution of the ancestral eutherian genome.
FAU - Deakin, Janine E
AU  - Deakin JE
AD  - ARC Centre of Excellence for Kangaroo Genomics, Canberra, Australia. 
      janine.deakin@canberra.edu.au.
FAU - Delbridge, Margaret L
AU  - Delbridge ML
FAU - Koina, Edda
AU  - Koina E
FAU - Harley, Nerida
AU  - Harley N
FAU - Alsop, Amber E
AU  - Alsop AE
FAU - Wang, Chenwei
AU  - Wang C
FAU - Patel, Vidushi S
AU  - Patel VS
FAU - Graves, Jennifer A Marshall
AU  - Graves JA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131121
PL  - England
TA  - BMC Evol Biol
JT  - BMC evolutionary biology
JID - 100966975
SB  - IM
MH  - Animals
MH  - *Biological Evolution
MH  - Chickens/genetics
MH  - Chromosome Mapping
MH  - Genome
MH  - Humans
MH  - Karyotype
MH  - Macropodidae/*genetics
MH  - Mammals/genetics
MH  - Marsupialia/*genetics
MH  - Opossums/*genetics
PMC - PMC4222502
EDAT- 2013/11/23 06:00
MHDA- 2014/02/20 06:00
PMCR- 2013/11/21
CRDT- 2013/11/23 06:00
PHST- 2013/09/26 00:00 [received]
PHST- 2013/11/19 00:00 [accepted]
PHST- 2013/11/23 06:00 [entrez]
PHST- 2013/11/23 06:00 [pubmed]
PHST- 2014/02/20 06:00 [medline]
PHST- 2013/11/21 00:00 [pmc-release]
AID - 1471-2148-13-258 [pii]
AID - 10.1186/1471-2148-13-258 [doi]
PST - epublish
SO  - BMC Evol Biol. 2013 Nov 21;13:258. doi: 10.1186/1471-2148-13-258.