PMID- 24262867
OWN - NLM
STAT- MEDLINE
DCOM- 20140912
LR  - 20140113
IS  - 1873-6971 (Electronic)
IS  - 0367-326X (Linking)
VI  - 92
DP  - 2014 Jan
TI  - Vascular barrier protective effects of pellitorine in LPS-induced inflammation in 
      vitro and in vivo.
PG  - 177-87
LID - S0367-326X(13)00301-8 [pii]
LID - 10.1016/j.fitote.2013.11.006 [doi]
AB  - Pellitorine (PT), an active amide compound, is well known to possess 
      insecticidal, antibacterial and anticancer properties. In this study, we first 
      investigated the possible barrier protective effects of pellitorine against 
      pro-inflammatory responses induced by lipopolysaccharide (LPS) and the associated 
      signaling pathways in vitro and in vivo. The barrier protective activities of PT 
      were determined by measuring permeability, monocyte adhesion and migration, and 
      activation of pro-inflammatory proteins in LPS-activated human umbilical vein 
      endothelial cells (HUVECs) and in mice. We found that PT inhibited LPS-induced 
      barrier disruption, expression of cell adhesion molecules (CAMs) and 
      adhesion/transendothelial migration of monocytes to human endothelial cells. PT 
      also suppressed LPS-induced hyperpermeability and leukocyte migration in vivo. 
      Further studies revealed that PT suppressed the production of tumor necrosis 
      factor-alpha (TNF-alpha) or Interleukin (IL)-6 and activation of nuclear factor-kappaB 
      (NF-kappaB) or extracellular regulated kinases (ERK) 1/2 by LPS. Moreover, treatment 
      with PT resulted in reduced LPS-induced lethal endotoxemia. These results suggest 
      that PT protects vascular barrier integrity by inhibiting hyperpermeability, 
      expression of CAMs, and adhesion and migration of leukocytes, thereby endorsing 
      its usefulness as a therapy for vascular inflammatory diseases.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Lee, Wonhwa
AU  - Lee W
AD  - College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, 
      Kyungpook National University, Daegu 702-701, Republic of Korea; Department of 
      Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, 
      Daegu 702-701, Republic of Korea.
FAU - Ku, Sae-Kwang
AU  - Ku SK
AD  - Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany 
      University, Gyeongsan 712-715, Republic of Korea.
FAU - Min, Byung-Woon
AU  - Min BW
AD  - Department of BioMedical Clinical Pathology, Hanlyo University, Gwangyang 
      545-704, Republic of Korea.
FAU - Lee, Sangkyu
AU  - Lee S
AD  - College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, 
      Kyungpook National University, Daegu 702-701, Republic of Korea.
FAU - Jee, Jun-Goo
AU  - Jee JG
AD  - College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, 
      Kyungpook National University, Daegu 702-701, Republic of Korea.
FAU - Kim, Jeong Ah
AU  - Kim JA
AD  - College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, 
      Kyungpook National University, Daegu 702-701, Republic of Korea.
FAU - Bae, Jong-Sup
AU  - Bae JS
AD  - College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, 
      Kyungpook National University, Daegu 702-701, Republic of Korea. Electronic 
      address: baejs@knu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131118
PL  - Netherlands
TA  - Fitoterapia
JT  - Fitoterapia
JID - 16930290R
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Interleukin-6)
RN  - 0 (NF-kappa B)
RN  - 0 (Plant Extracts)
RN  - 0 (Polyunsaturated Alkamides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 8IS5231171 (pellitorine)
SB  - IM
MH  - Animals
MH  - Asarum/*chemistry
MH  - Cell Adhesion/drug effects
MH  - Cell Adhesion Molecules/metabolism
MH  - Cell Movement/drug effects
MH  - Endothelial Cells/drug effects/metabolism
MH  - Endothelium, Vascular/cytology/drug effects/metabolism
MH  - Fatty Acids, Unsaturated/pharmacology/*therapeutic use
MH  - Human Umbilical Vein Endothelial Cells/*drug effects/metabolism
MH  - Humans
MH  - Inflammation/chemically induced/drug therapy/*metabolism
MH  - Inflammation Mediators/*metabolism
MH  - Interleukin-6/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Monocytes/metabolism
MH  - NF-kappa B/metabolism
MH  - Permeability
MH  - Phytotherapy
MH  - Plant Extracts/*pharmacology/therapeutic use
MH  - Polyunsaturated Alkamides/pharmacology/*therapeutic use
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Vascular Diseases/chemically induced/drug therapy/*metabolism
OTO - NOTNLM
OT  - Barrier integrity
OT  - Endothelium
OT  - Inflammation
OT  - Lipopolysaccharide
OT  - Pellitorine
EDAT- 2013/11/23 06:00
MHDA- 2014/09/13 06:00
CRDT- 2013/11/23 06:00
PHST- 2013/08/22 00:00 [received]
PHST- 2013/11/06 00:00 [revised]
PHST- 2013/11/09 00:00 [accepted]
PHST- 2013/11/23 06:00 [entrez]
PHST- 2013/11/23 06:00 [pubmed]
PHST- 2014/09/13 06:00 [medline]
AID - S0367-326X(13)00301-8 [pii]
AID - 10.1016/j.fitote.2013.11.006 [doi]
PST - ppublish
SO  - Fitoterapia. 2014 Jan;92:177-87. doi: 10.1016/j.fitote.2013.11.006. Epub 2013 Nov 
      18.