PMID- 24265869 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20131122 LR - 20211021 IS - 1976-9148 (Print) IS - 2005-4483 (Electronic) IS - 1976-9148 (Linking) VI - 21 IP - 3 DP - 2013 May 30 TI - Administration of Phytoceramide Enhances Memory and Upregulates the Expression of pCREB and BDNF in Hippocampus of Mice. PG - 229-33 LID - 10.4062/biomolther.2013.002 [doi] AB - This study was aimed at investigating the possible effects of phytoceramide (Pcer) on learning and memory and their underlying mechanisms. Phytoceramide was orally administered to ICR mice for 7 days. Memory performances were assessed using the passive avoidance test and Y-maze task. The expressions of phosphorylated cAMP response element binding protein (pCREB), brain-derived neurotrophic factor (BDNF) were measured with immunoblot. The incorporation of 5-bromo-2-deoxyuridine (BrdU) in hippocampal regions was investigated by using immunohistochemical methods. Treatment of Pcer enhanced cognitive performances in the passive avoidance test and Y-maze task. Immunoblotting studies revealed that the phosphorylated CREB and BDNF were significantly increased on hippocampus in the Pcer-treated mice. Immunohistochemical studies showed that the number of immunopositive cells to BrdU was significantly increased in the hippocampal dentate gyrus regions after Pcer-treatment for 7 days. These results suggest that Pcer contribute to enhancing memory and BDNF expression and it could be secondary to the elevation of neurogenesis. FAU - Lee, Yeonju AU - Lee Y AD - Department of Neuroscience and Tissue Injury Defense Research Center, School of Medicine, Ewha Womans University, Seoul 158-710, Republic of Korea. FAU - Kim, Jieun AU - Kim J FAU - Jang, Soyong AU - Jang S FAU - Oh, Seikwan AU - Oh S LA - eng PT - Journal Article PL - Korea (South) TA - Biomol Ther (Seoul) JT - Biomolecules & therapeutics JID - 101472832 PMC - PMC3830122 OTO - NOTNLM OT - Memory OT - Neurogenesis OT - Phytoceramide OT - Y-maze EDAT- 2013/11/23 06:00 MHDA- 2013/11/23 06:01 PMCR- 2013/05/30 CRDT- 2013/11/23 06:00 PHST- 2013/01/03 00:00 [received] PHST- 2013/03/19 00:00 [revised] PHST- 2013/03/27 00:00 [accepted] PHST- 2013/11/23 06:00 [entrez] PHST- 2013/11/23 06:00 [pubmed] PHST- 2013/11/23 06:01 [medline] PHST- 2013/05/30 00:00 [pmc-release] AID - ooomb4-21-229 [pii] AID - 10.4062/biomolther.2013.002 [doi] PST - ppublish SO - Biomol Ther (Seoul). 2013 May 30;21(3):229-33. doi: 10.4062/biomolther.2013.002.