PMID- 24266901
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20131209
LR  - 20211021
IS  - 1755-8166 (Print)
IS  - 1755-8166 (Electronic)
IS  - 1755-8166 (Linking)
VI  - 6
IP  - 1
DP  - 2013 Nov 22
TI  - Karyotype and cytogenetic mapping of 9 classes of repetitive DNAs in the genome 
      of the naked catfish Mystus bocourti (Siluriformes, Bagridae).
PG  - 51
LID - 10.1186/1755-8166-6-51 [doi]
AB  - BACKGROUND: In the present study, conventional and molecular cytogenetic studies 
      were performed in the naked catfish Mystus bocourti (Siluriformes, Bagridae). 
      Besides the conventional Giemsa staining, fluorescence in situ hybridization 
      (FISH) using nine classes of repetitive DNAs namely 5S and 18S rDNAs, U2 snRNA, 
      the microsatellites (CA)15 and (GA)15, telomeric repeats, and the 
      retrotransposable elements Rex1, 3 and 6. was also performed. RESULTS: M. 
      bocourti had 2n = 56 chromosomes with a karyotype composed by 11 m + 11 sm + 6 
      st/a and a fundamental number (NF) equal to 100 in both sexes. Heteromorphic sex 
      chromosome cannot be identified. The U2 snRNA, 5S and 18S rDNA were present in 
      only one pair of chromosomes but none of them in a syntenic position. 
      Microsatellites (CA)15 and (GA)15 showed hybridization signals at subtelomeric 
      regions of all chromosomes with a stronger accumulation into one specific 
      chromosomal pair. FISH with the telomeric probe revealed hybridization signals on 
      each telomere of all chromosomes and interstitial telomeric sites (ITS) were not 
      detected. The retrotransposable elements Rex1, 3 and 6 were generally spread 
      throughout the genome. CONCLUSIONS: In general, the repetitive sequences were not 
      randomly distributed in the genome, suggesting a pattern of compartmentalization 
      on the heterochromatic region of the chromosomes. Little is known about the 
      structure and organization of bagrid genomes and the knowledge of the chromosomal 
      distribution of repetitive DNA sequences in M. bocourti represents the first step 
      for achieving an integrated view of their genomes.
FAU - Supiwong, Weerayuth
AU  - Supiwong W
AD  - Department of Biology Faculty of Science, Khon Kaen University, 123 Mitraphap 
      Highway, Khon Kaen 40002, Muangkhonkaen District, Thailand. raccha@kku.ac.th.
FAU - Liehr, Thomas
AU  - Liehr T
FAU - Cioffi, Marcelo B
AU  - Cioffi MB
FAU - Chaveerach, Arunrat
AU  - Chaveerach A
FAU - Kosyakova, Nadezda
AU  - Kosyakova N
FAU - Pinthong, Krit
AU  - Pinthong K
FAU - Tanee, Tawatchai
AU  - Tanee T
FAU - Tanomtong, Alongklod
AU  - Tanomtong A
LA  - eng
PT  - Journal Article
DEP - 20131122
PL  - England
TA  - Mol Cytogenet
JT  - Molecular cytogenetics
JID - 101317942
PMC - PMC4176197
EDAT- 2013/11/26 06:00
MHDA- 2013/11/26 06:01
PMCR- 2013/11/22
CRDT- 2013/11/26 06:00
PHST- 2013/06/27 00:00 [received]
PHST- 2013/09/20 00:00 [accepted]
PHST- 2013/11/26 06:00 [entrez]
PHST- 2013/11/26 06:00 [pubmed]
PHST- 2013/11/26 06:01 [medline]
PHST- 2013/11/22 00:00 [pmc-release]
AID - 1755-8166-6-51 [pii]
AID - 10.1186/1755-8166-6-51 [doi]
PST - epublish
SO  - Mol Cytogenet. 2013 Nov 22;6(1):51. doi: 10.1186/1755-8166-6-51.