PMID- 24267259
OWN - NLM
STAT- MEDLINE
DCOM- 20140728
LR  - 20181202
IS  - 1879-1484 (Electronic)
IS  - 0021-9150 (Linking)
VI  - 231
IP  - 2
DP  - 2013 Dec
TI  - Impact of serum amyloid A on cellular cholesterol efflux to serum in type 2 
      diabetes mellitus.
PG  - 405-10
LID - S0021-9150(13)00608-4 [pii]
LID - 10.1016/j.atherosclerosis.2013.10.008 [doi]
AB  - OBJECTIVE: Serum amyloid A (SAA) is an acute phase response protein and has 
      apolipoprotein properties. Since type 2 diabetes is associated with chronic 
      subclinical inflammation, the objective of this study is to investigate the 
      changes in SAA level in type 2 diabetic patients and to evaluate the relationship 
      between SAA and the capacity of serum to induce cellular cholesterol efflux via 
      the two known cholesterol transporters, scavenger receptor class B type I (SR-BI) 
      and ATP-binding cassette transporter G1 (ABCG1). METHODS: 264 patients with type 
      2 diabetes mellitus (42% with normoalbuminuria, 30% microalbuminuria, and 28% 
      proteinuria) and 275 non-diabetic controls were recruited. SAA was measured by 
      ELISA. SR-BI and ABCG1-mediated cholesterol efflux to serum were determined by 
      measuring the transfer of [(3)H]cholesterol from Fu5AH rat hepatoma cells 
      expressing SR-BI and from human ABCG1-transfected CHO-K1 cells to the medium 
      containing the tested serum respectively. RESULTS: SAA was significantly 
      increased in diabetic patients with incipient or overt nephropathy. Both SR-BI 
      and ABCG1-mediated cholesterol efflux to serum were significantly impaired in all 
      three groups of diabetic patients (p < 0.01). SAA inversely correlated with 
      SR-BI-mediated cholesterol efflux (r = -0.36, p < 0.01) but did not correlate 
      with ABCG1-mediated cholesterol efflux. Stepwise linear regression analysis 
      showed that HDL, the presence or absence of diabetes, and log(SAA) were 
      significant independent determinants of SR-BI-mediated cholesterol efflux to 
      serum. CONCLUSION: SAA was increased in type 2 diabetic patients with incipient 
      or overt nephropathy, and SAA was associated with impairment of SR-BI-mediated 
      cholesterol efflux to serum.
CI  - Copyright (c) 2013 Elsevier Ireland Ltd. All rights reserved.
FAU - Tsun, J G S
AU  - Tsun JG
AD  - Department of Medicine, University of Hong Kong, Queen Mary Hospital, Pokfulam 
      Road, Hong Kong.
FAU - Shiu, S W M
AU  - Shiu SW
FAU - Wong, Y
AU  - Wong Y
FAU - Yung, S
AU  - Yung S
FAU - Chan, T M
AU  - Chan TM
FAU - Tan, K C B
AU  - Tan KC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131018
PL  - Ireland
TA  - Atherosclerosis
JT  - Atherosclerosis
JID - 0242543
RN  - 0 (ABCG1 protein, human)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily G, Member 1)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Apolipoproteins)
RN  - 0 (Cholesterol, HDL)
RN  - 0 (SCARB1 protein, human)
RN  - 0 (Scavenger Receptors, Class B)
RN  - 0 (Serum Amyloid A Protein)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily G, Member 1
MH  - ATP-Binding Cassette Transporters/*blood
MH  - Adult
MH  - Aged
MH  - Animals
MH  - Apolipoproteins/*blood
MH  - CHO Cells
MH  - Cell Line, Tumor
MH  - Cholesterol/*blood
MH  - Cholesterol, HDL/blood
MH  - Cricetinae
MH  - Cricetulus
MH  - Diabetes Complications/blood
MH  - Diabetes Mellitus, Type 2/*blood
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Linear Models
MH  - Male
MH  - Middle Aged
MH  - Rats
MH  - Scavenger Receptors, Class B/*blood
MH  - Serum Amyloid A Protein/*metabolism
OTO - NOTNLM
OT  - ATP-binding cassette transporter G1
OT  - Cellular cholesterol efflux
OT  - High density lipoprotein
OT  - Reverse cholesterol transport
OT  - Scavenger receptor class B type I
OT  - Serum amyloid A
EDAT- 2013/11/26 06:00
MHDA- 2014/07/30 06:00
CRDT- 2013/11/26 06:00
PHST- 2013/05/15 00:00 [received]
PHST- 2013/09/07 00:00 [revised]
PHST- 2013/10/10 00:00 [accepted]
PHST- 2013/11/26 06:00 [entrez]
PHST- 2013/11/26 06:00 [pubmed]
PHST- 2014/07/30 06:00 [medline]
AID - S0021-9150(13)00608-4 [pii]
AID - 10.1016/j.atherosclerosis.2013.10.008 [doi]
PST - ppublish
SO  - Atherosclerosis. 2013 Dec;231(2):405-10. doi: 
      10.1016/j.atherosclerosis.2013.10.008. Epub 2013 Oct 18.