PMID- 24267348
OWN - NLM
STAT- MEDLINE
DCOM- 20140630
LR  - 20211021
IS  - 1471-2180 (Electronic)
IS  - 1471-2180 (Linking)
VI  - 13
DP  - 2013 Nov 24
TI  - Escherichia coli YmdB regulates biofilm formation independently of its role as an 
      RNase III modulator.
PG  - 266
LID - 10.1186/1471-2180-13-266 [doi]
AB  - BACKGROUND: Ribonuclease III (RNase III) activity modulates hundreds of genes in 
      Escherichia coli (E. coli). YmdB, a member of the macrodomain protein family, is 
      one of known trans-acting regulators of RNase III activity; however, the 
      significance of its regulatory role in specific bacterial cellular processes and 
      related genes has not been determined. YmdB overexpression was used to model 
      YmdB-induced RNase III inhibition in vivo, and microarray analysis identified 
      gene targets and cellular processes related to RNase III inhibition. RESULTS: The 
      expression of >2,000 E. coli genes was modulated by YmdB induction; 129 genes 
      were strongly regulated, of which 80 have not been reported as RNase III targets. 
      Of these, ten are involved in biofilm formation. Significantly, YmdB 
      overexpression also inhibited biofilm formation via a process that is not 
      uniquely dependent upon RNase III inhibition. Moreover, biofilm formation is 
      interdependently regulated by RpoS, a known stress response regulator and biofilm 
      inhibitor, and by YmdB. CONCLUSIONS: This is the first global profile of target 
      genes modulated by YmdB-induced RNase III inhibition in E. coli, and the data 
      reveal a novel, hitherto unrecognized regulatory role for YmdB in biofilm 
      modulation.
FAU - Kim, Taeyeon
AU  - Kim T
AD  - Superbacteria Research Center, Korea Research Institute of Bioscience and 
      Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Korea. 
      sunny06@kribb.re.kr.
FAU - Lee, Juyeon
AU  - Lee J
FAU - Kim, Kwang-sun
AU  - Kim KS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131124
PL  - England
TA  - BMC Microbiol
JT  - BMC microbiology
JID - 100966981
RN  - 0 (Bacterial Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Sigma Factor)
RN  - 0 (YmdB protein, E coli)
RN  - 0 (sigma factor KatF protein, Bacteria)
RN  - EC 3.1.26.3 (Ribonuclease III)
SB  - IM
MH  - Bacterial Proteins/metabolism
MH  - Biofilms/*growth & development
MH  - Carrier Proteins/*metabolism
MH  - Escherichia coli/genetics/*physiology
MH  - Escherichia coli Proteins/*metabolism
MH  - Gene Expression
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation, Bacterial
MH  - Microarray Analysis
MH  - Ribonuclease III/*metabolism
MH  - Sigma Factor/metabolism
PMC - PMC4222554
EDAT- 2013/11/26 06:00
MHDA- 2014/07/01 06:00
PMCR- 2013/11/24
CRDT- 2013/11/26 06:00
PHST- 2013/06/01 00:00 [received]
PHST- 2013/11/21 00:00 [accepted]
PHST- 2013/11/26 06:00 [entrez]
PHST- 2013/11/26 06:00 [pubmed]
PHST- 2014/07/01 06:00 [medline]
PHST- 2013/11/24 00:00 [pmc-release]
AID - 1471-2180-13-266 [pii]
AID - 10.1186/1471-2180-13-266 [doi]
PST - epublish
SO  - BMC Microbiol. 2013 Nov 24;13:266. doi: 10.1186/1471-2180-13-266.