PMID- 24268109
OWN - NLM
STAT- MEDLINE
DCOM- 20141001
LR  - 20140203
IS  - 1878-3279 (Electronic)
IS  - 0171-2985 (Linking)
VI  - 219
IP  - 3
DP  - 2014 Mar
TI  - Chemokines and other GPCR ligands synergize in receptor-mediated migration of 
      monocyte-derived immature and mature dendritic cells.
PG  - 218-29
LID - S0171-2985(13)00187-3 [pii]
LID - 10.1016/j.imbio.2013.10.004 [doi]
AB  - Dendritic cells (DCs) are potent antigen presenting cells, described as the 
      initiators of adaptive immune responses. Immature monocyte-derived DCs (MDDC) 
      showed decreased CD14 expression, increased cell surface markers DC-SIGN and CD1a 
      and enhanced levels of receptors for the chemokines CCL3 (CCR1/CCR5) and CXCL8 
      (CXCR1/CXCR2) compared with human CD14(+) monocytes. After further MDDC maturation 
      by LPS, the markers CD80 and CD83 and the chemokine receptors CXCR4 and CCR7 were 
      upregulated, whereas CCR1, CCR2 and CCR5 expression was reduced. CCL3 
      dose-dependently synergized with CXCL8 or CXCL12 in chemotaxis of immature MDDC. 
      CXCL12 augmented the CCL3-induced ERK1/2 and Akt phosphorylation in immature 
      MDDC, although the synergy between CCL3 and CXCL12 in chemotaxis of immature MDDC 
      was dependent on the Akt signaling pathway but not on ERK1/2 phosphorylation. 
      CCL2 also synergized with CXCL12 in immature MDDC migration. Moreover, two CXC 
      chemokines not sharing receptors (CXCL12 and CXCL8) cooperated in immature MDDC 
      chemotaxis, whereas two CC chemokines (CCL3 and CCL7) sharing CCR1 did not. 
      Further, the non-chemokine G protein-coupled receptor ligands chemerin and fMLP 
      synergized with respectively CCL7 and CCL3 in immature MDDC signaling and 
      migration. Finally, CXCL12 and CCL3 did not cooperate, but CXCL12 synergized with 
      CCL21 in mature MDDC chemotaxis. Thus, chemokine synergy in immature and mature 
      MDDC migration is dose-dependently regulated by chemokines via alterations in 
      their chemokine receptor expression pattern according to their role in immune 
      responses.
CI  - Copyright (c) 2013 Elsevier GmbH. All rights reserved.
FAU - Gouwy, Mieke
AU  - Gouwy M
AD  - Laboratory of Molecular Immunology, Rega Institute for Medical Research, 
      Department of Microbiology and Immunology, University of Leuven, Leuven, Belgium.
FAU - Struyf, Sofie
AU  - Struyf S
AD  - Laboratory of Molecular Immunology, Rega Institute for Medical Research, 
      Department of Microbiology and Immunology, University of Leuven, Leuven, Belgium.
FAU - Leutenez, Lien
AU  - Leutenez L
AD  - Laboratory of Molecular Immunology, Rega Institute for Medical Research, 
      Department of Microbiology and Immunology, University of Leuven, Leuven, Belgium.
FAU - Portner, Noemie
AU  - Portner N
AD  - Laboratory of Molecular Immunology, Rega Institute for Medical Research, 
      Department of Microbiology and Immunology, University of Leuven, Leuven, Belgium.
FAU - Sozzani, Silvano
AU  - Sozzani S
AD  - Department of Molecular and Translational Medicine, University of Brescia, Viale 
      Europa 11, 25123 Brescia, Italy; Humanitas Clinical and Research Center, Rozzano, 
      Italy.
FAU - Van Damme, Jo
AU  - Van Damme J
AD  - Laboratory of Molecular Immunology, Rega Institute for Medical Research, 
      Department of Microbiology and Immunology, University of Leuven, Leuven, Belgium. 
      Electronic address: jo.vandamme@rega.kuleuven.be.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131014
PL  - Netherlands
TA  - Immunobiology
JT  - Immunobiology
JID - 8002742
RN  - 0 (Antigens, CD)
RN  - 0 (Chemokines)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - EC 2.7.11.1 (Oncogene Protein v-akt)
SB  - IM
MH  - Adaptive Immunity
MH  - Antigens, CD/metabolism
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Chemokines/*metabolism
MH  - Chemotaxis
MH  - Dendritic Cells/*immunology
MH  - Humans
MH  - Immunity, Innate
MH  - Monocytes/*immunology
MH  - Oncogene Protein v-akt/metabolism
MH  - Receptor Cross-Talk
MH  - Receptors, Chemokine/genetics/*metabolism
MH  - Receptors, G-Protein-Coupled/genetics/*metabolism
MH  - Signal Transduction
OTO - NOTNLM
OT  - BCA
OT  - CAM
OT  - CC chemokine ligand
OT  - CCL
OT  - CI
OT  - CXC chemokine ligand
OT  - CXCL
OT  - Chemoattractants
OT  - ERK
OT  - G protein-coupled receptor
OT  - GAG
OT  - GM-CSF
OT  - GPCR
OT  - LPS
OT  - MACS
OT  - MAPK
OT  - MDDC
OT  - MFI
OT  - Maturation
OT  - Migration
OT  - PBMC
OT  - PHA
OT  - Receptor expression
OT  - SEM
OT  - Signal transduction
OT  - Synergy
OT  - bicinchoninic acid
OT  - cell-adhesion molecules
OT  - chemotactic index
OT  - extracellular signal-regulated kinase
OT  - glycosaminoglycans
OT  - granulocyte-macrophage colony stimulating factor
OT  - lipopolysaccharide
OT  - magnetic cell sorting
OT  - mean fluorescence intensity
OT  - mitogen-activated protein kinase
OT  - monocyte-derived dendritic cells
OT  - peripheral blood mononuclear cells
OT  - phytohemagglutinin
OT  - standard error of the mean
EDAT- 2013/11/26 06:00
MHDA- 2014/10/02 06:00
CRDT- 2013/11/26 06:00
PHST- 2013/05/22 00:00 [received]
PHST- 2013/09/27 00:00 [revised]
PHST- 2013/10/07 00:00 [accepted]
PHST- 2013/11/26 06:00 [entrez]
PHST- 2013/11/26 06:00 [pubmed]
PHST- 2014/10/02 06:00 [medline]
AID - S0171-2985(13)00187-3 [pii]
AID - 10.1016/j.imbio.2013.10.004 [doi]
PST - ppublish
SO  - Immunobiology. 2014 Mar;219(3):218-29. doi: 10.1016/j.imbio.2013.10.004. Epub 
      2013 Oct 14.