PMID- 2426842
OWN - NLM
STAT- MEDLINE
DCOM- 19860916
LR  - 20190727
IS  - 0040-8727 (Print)
IS  - 0040-8727 (Linking)
VI  - 148
IP  - 4
DP  - 1986 Apr
TI  - Relevance of anti-alpha-fetoprotein antibody for radioimmunodetection: analysis 
      with alpha-fetoprotein-secreting rat hepatoma and antigen-bound acrylic beads.
PG  - 341-51
AB  - Using AFP-secreting AH66 rat hepatoma cells and AFP-bound acrylic beads (AFP-B), 
      artificial cells expressing surface antigen, the effect of blood AFP level on the 
      accumulation of radiolabeled rabbit anti-AFP antibody in the site of tumor and 
      beads implanted in rats was examined. Scintigrams of hepatoma-bearing rats with 
      implanted AFP-B showed a marked localization in AFP-B site, but not in the tumor 
      site on day 5 after the injection of radiolabeled antibody. The imaging of AFP-B 
      was most satisfactory in normal rats with extremely low blood AFP. Tissue/blood 
      radioactivity ratio in AFP-B on day 5 was found to be significantly higher in 
      normal rats (17.43 +/- 5.55), compared with 6.66 +/- 1.67 in the tumor-bearing 
      rats (p less than 0.01), while the ratio of AFP-B in tumor-bearing rats 
      remarkably recovered to 11.20 +/- 0.25 (p less than 0.01) after whole blood 
      exchange to reduce an elevated serum AFP level. The accumulation of anti-AFP 
      antibody in the tumor was not observed irrespective of AFP-B. 
      Microautoradiography of tissue sections showed the specific localization of 
      radioactivity on the surface of AFP-B but not on the surface or the inside of the 
      tumor cells. Immune complexes were detected in plasma of tumor-bearing rats 1 min 
      to 7 days after the administration of the radiolabeled antibody. In conclusion, 
      the application of anti-AFP antibody for the diagnosis and treatment of tumors is 
      limited to the case of the expression of AFP on the surface of tumor cells and 
      lower AFP level in circulation.
FAU - Tanno, H
AU  - Tanno H
FAU - Owada, Y
AU  - Owada Y
FAU - Sato, T
AU  - Sato T
FAU - Koyama, K
AU  - Koyama K
FAU - Tachibana, T
AU  - Tachibana T
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Tohoku J Exp Med
JT  - The Tohoku journal of experimental medicine
JID - 0417355
RN  - 0 (Antibodies, Neoplasm)
RN  - 0 (Antigen-Antibody Complex)
RN  - 0 (Antigens, Surface)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (alpha-Fetoproteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Neoplasm/analysis/*immunology
MH  - Antigen-Antibody Complex/analysis
MH  - Antigens, Surface/immunology
MH  - Liver Neoplasms, Experimental/*immunology
MH  - Male
MH  - Neoplasm Proteins/immunology
MH  - Rats
MH  - alpha-Fetoproteins/analysis/*immunology
EDAT- 1986/04/01 00:00
MHDA- 1986/04/01 00:01
CRDT- 1986/04/01 00:00
PHST- 1986/04/01 00:00 [pubmed]
PHST- 1986/04/01 00:01 [medline]
PHST- 1986/04/01 00:00 [entrez]
AID - 10.1620/tjem.148.341 [doi]
PST - ppublish
SO  - Tohoku J Exp Med. 1986 Apr;148(4):341-51. doi: 10.1620/tjem.148.341.