PMID- 24268486
OWN - NLM
STAT- MEDLINE
DCOM- 20150406
LR  - 20171116
IS  - 1532-2971 (Electronic)
IS  - 1090-0233 (Linking)
VI  - 199
IP  - 1
DP  - 2014 Jan
TI  - Influence of prostaglandin F(2)alpha analogues on the secretory function of bovine 
      luteal cells and ovarian arterial contractility in vitro.
PG  - 131-7
LID - S1090-0233(13)00449-8 [pii]
LID - 10.1016/j.tvjl.2013.09.021 [doi]
AB  - Although prostaglandin (PG) F2alpha analogues are routinely used for oestrus 
      synchronisation in cattle, their effects on the function of the bovine corpus 
      luteum (CL), and on ovarian arterial contractility, may not reflect the 
      physiological effects of endogenous PGF2alpha. In the first of two related 
      experiments, the effects of different analogues of PGF2alpha (aPGF2alpha) on the 
      secretory function and apoptosis of cultured bovine cells of the CL were 
      assessed. Enzymatically-isolated bovine luteal cells (from between days 8 and 12 
      of the oestrous cycle), were stimulated for 24h with naturally-occurring PGF2alpha or 
      aPGF2alpha (dinoprost, cloprostenol or luprostiol). Secretion of progesterone (P4) 
      was determined and cellular [Ca(2+)]i mobilisation, as well as cell viability and 
      apoptosis were measured. Naturally-occurring PGF2alpha and dinoprost stimulated P4 
      secretion (P<0.05), whereas cloprostenol and luprostiol did not influence P4 
      synthesis. The greatest cytotoxic and pro-apoptotic effects were observed in the 
      luprostiol-treated cells, at 37.3% and 202%, respectively (P<0.001). The greatest 
      effect on [Ca(2+)]i mobilisation in luteal cells was observed post-luprostiol 
      treatment (200%; P<0.001). In a second experiment, the influence of 
      naturally-occurring PGF2alpha and aPGF2alpha on ovarian arterial contraction in vitro, 
      were examined. No differences in the effects of dinoprost or naturally-occurring 
      PGF2alpha were found across the studied parameters. The effects of cloprostenol and 
      luprostiol on luteal cell death, in addition to their effects on ovarian arterial 
      contractility, were much greater than those produced by treatment with 
      naturally-occurring PGF2alpha.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Korzekwa, A J
AU  - Korzekwa AJ
AD  - Department of Reproductive Immunology and Pathology, Institute of Animal 
      Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, 
      Poland.
FAU - Lukasik, K
AU  - Lukasik K
AD  - Department of Reproductive Immunology and Pathology, Institute of Animal 
      Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, 
      Poland; Department of Pharmacology and Toxicology, Faculty of Veterinary 
      Medicine, University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn, Poland; 
      Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and 
      Technology, Okayama University, Tsushima Naka 1-1-1, Okayama 700-8530, Japan.
FAU - Pilawski, W
AU  - Pilawski W
AD  - Department of Reproductive Immunology and Pathology, Institute of Animal 
      Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, 
      Poland.
FAU - Piotrowska-Tomala, K K
AU  - Piotrowska-Tomala KK
AD  - Department of Reproductive Immunology and Pathology, Institute of Animal 
      Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, 
      Poland.
FAU - Jaroszewski, J J
AU  - Jaroszewski JJ
AD  - Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, 
      University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn, Poland.
FAU - Yoshioka, S
AU  - Yoshioka S
AD  - Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and 
      Technology, Okayama University, Tsushima Naka 1-1-1, Okayama 700-8530, Japan.
FAU - Okuda, K
AU  - Okuda K
AD  - Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and 
      Technology, Okayama University, Tsushima Naka 1-1-1, Okayama 700-8530, Japan.
FAU - Skarzynski, D J
AU  - Skarzynski DJ
AD  - Department of Reproductive Immunology and Pathology, Institute of Animal 
      Reproduction and Food Research, Polish Academy of Sciences, 10-747 Olsztyn, 
      Poland. Electronic address: d.skarzynski@pan.olsztyn.pl.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130926
PL  - England
TA  - Vet J
JT  - Veterinary journal (London, England : 1997)
JID - 9706281
RN  - 0 (Prostaglandins F, Synthetic)
RN  - 4208238832 (Cloprostenol)
RN  - B7IN85G1HY (Dinoprost)
RN  - HWR60H5GZB (luprostenol)
SB  - IM
EIN - Vet J. 2015 May;204(2):235
MH  - Animals
MH  - Arteries/*drug effects
MH  - *Cattle
MH  - Cloprostenol/pharmacology
MH  - Dinoprost/*analogs & derivatives/pharmacology
MH  - Female
MH  - Luteal Cells/*drug effects/physiology
MH  - Ovary/*blood supply
MH  - Prostaglandins F, Synthetic/pharmacology
MH  - Vasoconstriction/*drug effects
OTO - NOTNLM
OT  - Arterial contraction
OT  - Cattle
OT  - Corpus luteum
OT  - Luteolysis
OT  - PGF(2alpha)
OT  - Prostaglandin F(2alpha)
EDAT- 2013/11/26 06:00
MHDA- 2015/04/07 06:00
CRDT- 2013/11/26 06:00
PHST- 2012/12/27 00:00 [received]
PHST- 2013/09/15 00:00 [revised]
PHST- 2013/09/17 00:00 [accepted]
PHST- 2013/11/26 06:00 [entrez]
PHST- 2013/11/26 06:00 [pubmed]
PHST- 2015/04/07 06:00 [medline]
AID - S1090-0233(13)00449-8 [pii]
AID - 10.1016/j.tvjl.2013.09.021 [doi]
PST - ppublish
SO  - Vet J. 2014 Jan;199(1):131-7. doi: 10.1016/j.tvjl.2013.09.021. Epub 2013 Sep 26.