PMID- 24269709
OWN - NLM
STAT- MEDLINE
DCOM- 20140926
LR  - 20140113
IS  - 1096-0309 (Electronic)
IS  - 0003-2697 (Linking)
VI  - 447
DP  - 2014 Feb 15
TI  - Quantitative analysis of heterocyclic amines in urine by liquid chromatography 
      coupled with tandem mass spectrometry.
PG  - 169-76
LID - S0003-2697(13)00548-4 [pii]
LID - 10.1016/j.ab.2013.11.012 [doi]
AB  - A sensitive, reproducible, and rapid analytical method for the analysis of 
      trace-level heterocyclic amines (HCAs) that are expected to have high levels of 
      human exposure was developed. Liquid-liquid extraction (LLE) with dichloromethane 
      (DCM) followed by solid-phase extraction (SPE) was carried out. Liquid extraction 
      with DCM under basic conditions was efficient in extracting HCAs from urine 
      samples. For further purification, mixed mode cationic exchange (MCX) cartridges 
      were applied to eliminate the remaining interferences after liquid extraction. 
      Separation and quantification were performed by liquid chromatography coupled 
      with tandem mass spectrometry (LC-MS/MS) in selected reaction monitoring (SRM) 
      mode. The overall recoveries ranged between 71.0% and 113.6% with relative 
      standard deviations (RSDs) of 5.1% to 14.7% for the entire procedure. The limits 
      of detection (LODs) and limits of quantification (LOQs) of the proposed 
      analytical method were in the ranges of 0.04 to 0.10 ng/ml and 0.15 to 0.36 
      ng/ml, respectively. This method was applied to the analysis of monitoring in 
      urine samples for Korean school children, and the results demonstrated that the 
      method can be used for the trace determination of HCAs in urine samples.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Shin, Jeoung Hwa
AU  - Shin JH
AD  - Seoul Center, Korea Basic Science Institute, Sungbuk-Ku, Seoul 136-701, Republic 
      of Korea.
FAU - Na, Yun-Cheol
AU  - Na YC
AD  - Seoul Center, Korea Basic Science Institute, Sungbuk-Ku, Seoul 136-701, Republic 
      of Korea.
FAU - Chung, Joo Hee
AU  - Chung JH
AD  - Seoul Center, Korea Basic Science Institute, Sungbuk-Ku, Seoul 136-701, Republic 
      of Korea.
FAU - Gorinstein, Shela
AU  - Gorinstein S
AD  - Institute for Drug Research, School of Pharmacy, The Hebrew University, Hadassah 
      Medical School, Jerusalem 91120, Israel.
FAU - Ahn, Yun Gyong
AU  - Ahn YG
AD  - Seoul Center, Korea Basic Science Institute, Sungbuk-Ku, Seoul 136-701, Republic 
      of Korea. Electronic address: ygahn@kbsi.re.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131121
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 0 (Amines)
RN  - 0 (Heterocyclic Compounds)
RN  - 0 (Mutagens)
SB  - IM
MH  - Amines/*chemistry/*toxicity
MH  - Child
MH  - Chromatography, Liquid
MH  - Environmental Exposure/analysis
MH  - Heterocyclic Compounds/*chemistry
MH  - Humans
MH  - Mutagens/*analysis/*chemistry
MH  - Risk Assessment
MH  - Tandem Mass Spectrometry
MH  - Urinalysis/*methods
OTO - NOTNLM
OT  - Heterocyclic amines
OT  - LC-MS/MS
OT  - LLE
OT  - Solid-phase extraction
OT  - Urine
EDAT- 2013/11/26 06:00
MHDA- 2014/09/27 06:00
CRDT- 2013/11/26 06:00
PHST- 2013/09/24 00:00 [received]
PHST- 2013/11/05 00:00 [revised]
PHST- 2013/11/09 00:00 [accepted]
PHST- 2013/11/26 06:00 [entrez]
PHST- 2013/11/26 06:00 [pubmed]
PHST- 2014/09/27 06:00 [medline]
AID - S0003-2697(13)00548-4 [pii]
AID - 10.1016/j.ab.2013.11.012 [doi]
PST - ppublish
SO  - Anal Biochem. 2014 Feb 15;447:169-76. doi: 10.1016/j.ab.2013.11.012. Epub 2013 
      Nov 21.