PMID- 24269777 OWN - NLM STAT- MEDLINE DCOM- 20140908 LR - 20220318 IS - 1872-7573 (Electronic) IS - 0378-8741 (Linking) VI - 151 IP - 1 DP - 2014 TI - The anti-inflammation effect of Moutan Cortex on advanced glycation end products-induced rat mesangial cells dysfunction and High-glucose-fat diet and streptozotocin-induced diabetic nephropathy rats. PG - 591-600 LID - S0378-8741(13)00809-X [pii] LID - 10.1016/j.jep.2013.11.015 [doi] AB - ETHNOPHARMACOLOGICAL RELEVANCE: Moutan Cortex (MC, family: Paeonia suffruticosa Andr.) is a well-known traditional herbal medicine that has been shown to hold a protective effect on inflammation in several diseases. However, its anti-inflammatory activity on diabetic nephropathy (DN) has been less reported. The present study was conducted to evaluate the potential attenuation activities of MC on inflammation in AGEs-induced rat mesangial cells dysfunction and high-glucose-fat diet and streptozotocin (STZ)-induced DN rats and explore the possible mechanism underlying its DN effect. MATERIALS AND METHODS: The inflammation in mesangial cells (HBZY-1) was induced by 200 mug/ml advanced glycation end products (AGEs). DN rats model was established by an administration high-glucose-fat diet and an intraperitoneal injection of STZ (30 mg/kg). Interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) level in cell supernatant and rats serum were detected by appropriate kits. A co-culture system of mesangial cells and macrophages was performed to evaluate the migration of macrophages. Immunohistochemical assay was applied to examine transforming growth factor beta1 (TGF-beta1), IL-6, MCP-1 and intercellular adhesion molecule-1 (ICAM-1) expression in kidney tissues of rats. Furthermore, western blot analysis was carried out to examine TGF-beta1, IL-6, MCP-1, ICAM-1 and RAGE protein expressions in mesangial cells. RESULTS: Pretreatment with MC could significantly inhibit AGEs-induced migration of macrophages in the co-culture system of mesangial cell and macrophage. MC could decrease IL-6 and MCP-1 levels in serum of DN rats in a dose-dependent manner. Furthermore, MC also improved the blood glucose, serum creatinine and urine protein levels. Both immunocytochemistry analysis and western blot analysis showed that MC decreased significantly the over-expression of IL-6, MCP-1, TGF-beta1, ICAM-1 and RAGE in mesangial cells or kidney tissues. Additionally, the protein expression of proinflammatory cytokine could also be down-regulated by the pretreatment of RAGE-Ab (5 mug/ml). CONCLUSION: These findings indicated that the extract of MC had an amelioration activity on the inflammation in AGEs-induced mesangial cells dysfunction and high-glucose-fat diet and STZ-induced DN rats. The protective effect might be associated with the intervention of MC via target of RAGE. These findings suggested that MC might be a benefit agent for the prevention and treatment of DN. CI - (c) 2013 Elsevier Ireland Ltd. All rights reserved. FAU - Zhang, Ming-hua AU - Zhang MH AD - Key Laboratory of New Drug Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu, Nanjing 210028, PR China; Department of Pharmaceutics, Jiangsu University, Jiangsu, Zhenjiang 212013, PR China. FAU - Feng, Liang AU - Feng L AD - Key Laboratory of New Drug Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu, Nanjing 210028, PR China. Electronic address: wenmoxiushi@163.com. FAU - Zhu, Mao-mao AU - Zhu MM AD - Key Laboratory of New Drug Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu, Nanjing 210028, PR China; Nanjing Institute of Supervision & Testing on Product Quality, Jiangsu, Nanjing 210028, PR China. FAU - Gu, Jun-fei AU - Gu JF AD - Key Laboratory of New Drug Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu, Nanjing 210028, PR China; College of Pharmacy, Nanjing University of Chinese Medicine, Jiangsu, Nanjing 210046, PR China. FAU - Jiang, Jun AU - Jiang J AD - Key Laboratory of New Drug Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu, Nanjing 210028, PR China; Nanjing Institute of Supervision & Testing on Product Quality, Jiangsu, Nanjing 210028, PR China; College of Pharmacy, Nanjing University of Chinese Medicine, Jiangsu, Nanjing 210046, PR China. FAU - Cheng, Xu-dong AU - Cheng XD AD - Key Laboratory of New Drug Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu, Nanjing 210028, PR China; College of Pharmacy, Nanjing University of Chinese Medicine, Jiangsu, Nanjing 210046, PR China. FAU - Ding, Shu-ming AU - Ding SM AD - Key Laboratory of New Drug Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu, Nanjing 210028, PR China; College of Pharmacy, Nanjing University of Chinese Medicine, Jiangsu, Nanjing 210046, PR China. FAU - Wu, Chan AU - Wu C AD - Key Laboratory of New Drug Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu, Nanjing 210028, PR China; College of Pharmacy, Nanjing University of Chinese Medicine, Jiangsu, Nanjing 210046, PR China. FAU - Jia, Xiao-bin AU - Jia XB AD - Key Laboratory of New Drug Delivery Systems of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu, Nanjing 210028, PR China; Department of Pharmaceutics, Jiangsu University, Jiangsu, Zhenjiang 212013, PR China; College of Pharmacy, Nanjing University of Chinese Medicine, Jiangsu, Nanjing 210046, PR China. Electronic address: jxiaobin2005@hotmail.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20131121 PL - Ireland TA - J Ethnopharmacol JT - Journal of ethnopharmacology JID - 7903310 RN - 0 (Anti-Inflammatory Agents) RN - 0 (Dietary Fats) RN - 0 (Drugs, Chinese Herbal) RN - 0 (Glycation End Products, Advanced) RN - 0 (moutan cortex) RN - IY9XDZ35W2 (Glucose) SB - IM MH - Animals MH - Anti-Inflammatory Agents/chemistry/pharmacology MH - Cell Line MH - Diabetes Mellitus, Experimental/*complications MH - Diabetic Nephropathies/drug therapy MH - Dietary Fats/*adverse effects MH - Dose-Response Relationship, Drug MH - Drugs, Chinese Herbal/chemistry/*pharmacology MH - Gene Expression Regulation/drug effects MH - Glucose/administration & dosage/*adverse effects MH - Glycation End Products, Advanced/*toxicity MH - Mesangial Cells/*drug effects/metabolism MH - Paeonia/chemistry MH - Random Allocation MH - Rats OTO - NOTNLM OT - AG OT - AGEs OT - BSA OT - DMEM OT - DN OT - Diabetic nephropathy OT - Dulbecco's modi fi ed Eagle's medium OT - FBS OT - ICAM-1 OT - IL-6 OT - Inflammation OT - MC OT - MCP-1 OT - Moutan Cortex OT - Moutan cortex OT - OD OT - OS OT - PBS OT - RAGE OT - ROS OT - Rage OT - SD OT - STZ OT - TGF-beta1 OT - advanced glycation end products OT - aminoguanidine OT - bovine serum albumin OT - diabetic nephropathy OT - fetal bovine serum OT - intercellular adhesion molecule-1 OT - interleukin-6 OT - monocyte chemoattractant protein-1 OT - optical density OT - oxidative stress OT - phosphate buffered saline OT - reactive oxygen species OT - receptor for AGEs OT - standard deviation OT - streptozotocin OT - transforming growth factor beta1 EDAT- 2013/11/26 06:00 MHDA- 2014/09/10 06:00 CRDT- 2013/11/26 06:00 PHST- 2013/03/14 00:00 [received] PHST- 2013/08/15 00:00 [revised] PHST- 2013/11/10 00:00 [accepted] PHST- 2013/11/26 06:00 [entrez] PHST- 2013/11/26 06:00 [pubmed] PHST- 2014/09/10 06:00 [medline] AID - S0378-8741(13)00809-X [pii] AID - 10.1016/j.jep.2013.11.015 [doi] PST - ppublish SO - J Ethnopharmacol. 2014;151(1):591-600. doi: 10.1016/j.jep.2013.11.015. Epub 2013 Nov 21.