PMID- 24270314
OWN - NLM
STAT- MEDLINE
DCOM- 20140826
LR  - 20211021
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 9
IP  - 2
DP  - 2014 Feb
TI  - Protective effect of ginsenoside Rg1 on lidocaine-induced apoptosis.
PG  - 395-400
LID - 10.3892/mmr.2013.1822 [doi]
AB  - Lidocaine, as an anesthetic substance, is often used for surface and spinal 
      anesthesia. However, studies have shown that lidocaine may induce transient 
      neurological symptoms and cauda equina syndrome. In the present study the effects 
      of the ginsenoside Rg1 (Rg1) on lidocaine‑induced apoptosis were assessed in 
      Jurkat cells using flow cytometry and terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL). The data showed that 
      incubation with Rg1 provides protection against lidocaine‑induced apoptosis in 
      cultured Jurkat cells. In order to investigate the effect of Rg1 on the apoptosis 
      pathway, caspase 3 gene expression was determined. The results suggested that the 
      protective effect of Rg1 on lidocaine‑induced apoptosis is mediated by altering 
      the level of B‑cell lymphoma‑2 (BCL‑2) family proteins and downregulating 
      caspase‑3 expression. In conclusion, the present study demonstrated that 
      incubation with Rg1 provides protection against lidocaine‑induced apoptosis in 
      cultured Jurkat cells. In addition, the study demonstrated that Rg1 is a notable 
      antiapoptotic molecule that is capable of blocking the caspase‑dependent 
      signaling cascade in Jurkat cells, and that the protective effect of Rg1 on 
      lidocaine‑induced apoptosis is mediated by altering levels of BCL‑2 family 
      proteins and downregulating caspase‑3 expression. The present study provides the 
      basis for understanding and evaluating the effect of Rg1 in the in vivo treatment 
      of lidocaine-induced transient neurological symptoms and cauda equina syndrome by 
      lidocaine.
FAU - Li, Hui
AU  - Li H
AD  - Department of Anesthesiology, Second Xiangya Hospital, Central South University, 
      Changsha, Hunan 410011, P.R. China.
FAU - Xu, Junmei
AU  - Xu J
AD  - Department of Anesthesiology, Second Xiangya Hospital, Central South University, 
      Changsha, Hunan 410011, P.R. China.
FAU - Wang, Xin
AU  - Wang X
AD  - Department of Anesthesiology, Second Xiangya Hospital, Central South University, 
      Changsha, Hunan 410011, P.R. China.
FAU - Yuan, Guixiu
AU  - Yuan G
AD  - Department of Anesthesiology, Second Xiangya Hospital, Central South University, 
      Changsha, Hunan 410011, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20131121
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Ginsenosides)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 98PI200987 (Lidocaine)
RN  - EC 3.4.22.- (Caspase 3)
RN  - PJ788634QY (ginsenoside Rg1)
SB  - IM
MH  - Apoptosis/drug effects
MH  - Caspase 3/biosynthesis
MH  - Cell Survival/drug effects
MH  - Gene Expression Regulation
MH  - Ginsenosides/*administration & dosage
MH  - Humans
MH  - Jurkat Cells
MH  - Lidocaine/administration & dosage/*adverse effects
MH  - Neurons/drug effects
MH  - Polyradiculopathy/chemically induced/*drug therapy/pathology
MH  - Proto-Oncogene Proteins c-bcl-2/biosynthesis
PMC - PMC3896509
EDAT- 2013/11/26 06:00
MHDA- 2014/08/27 06:00
PMCR- 2013/11/21
CRDT- 2013/11/26 06:00
PHST- 2013/06/06 00:00 [received]
PHST- 2013/10/31 00:00 [accepted]
PHST- 2013/11/26 06:00 [entrez]
PHST- 2013/11/26 06:00 [pubmed]
PHST- 2014/08/27 06:00 [medline]
PHST- 2013/11/21 00:00 [pmc-release]
AID - mmr-09-02-0395 [pii]
AID - 10.3892/mmr.2013.1822 [doi]
PST - ppublish
SO  - Mol Med Rep. 2014 Feb;9(2):395-400. doi: 10.3892/mmr.2013.1822. Epub 2013 Nov 21.