PMID- 24274767
OWN - NLM
STAT- MEDLINE
DCOM- 20150415
LR  - 20151119
IS  - 1520-5827 (Electronic)
IS  - 0743-7463 (Linking)
VI  - 29
IP  - 50
DP  - 2013 Dec 17
TI  - Low temperature thermal dependent Filgrastim adsorption behavior detected with 
      ToF-SIMS.
PG  - 15573-8
LID - 10.1021/la403607m [doi]
AB  - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) detected changes in 
      Filgrastim (granulocyte colony stimulating growth factor, G-CSF) adsorption 
      behavior at a solid interface when exposed to temperatures as low as 35  degrees C, i.e., 
      before thermal denaturation, was detected by circular dichroism (CD) or dynamic 
      light scattering (DLS). Biopharmaceuticals rely on maintaining sufficient 
      conformation to impart correct biological function in vivo. Stability of such 
      molecules is critical during synthesis, storage, transport, and administration. 
      CD analysis indicated loss of structure at temperatures greater than ~60  degrees C, 
      while DLS detected aggregation at ~42  degrees C. Furthermore, we demonstrate the nature 
      of G-CSF interaction with a surface was altered rapidly and at relatively low 
      temperatures. Specifically, after 10 min thermal treatment, changes in adsorption 
      behavior occurred at 35  degrees C indicated by principal component analysis of spectra 
      as primarily due to increasing yields of methionine fragments. This was likely to 
      be due to either altering the preferential protein orientation upon adsorption or 
      greater denaturation exposing the hydrophobic core. This investigation 
      demonstrates the sensitivity of ToF-SIMS in studying biopharmaceutical adsorption 
      and conformational change and can assist with studies into promoting their 
      stability.
FAU - Kempson, Ivan M
AU  - Kempson IM
AD  - Ian Wark Research Institute, University of South Australia , Mawson Lakes, S.A. 
      5095, Australia.
FAU - Chang, Patrick
AU  - Chang P
FAU - Bremmell, Kristen
AU  - Bremmell K
FAU - Prestidge, Clive A
AU  - Prestidge CA
LA  - eng
PT  - Journal Article
DEP - 20131204
PL  - United States
TA  - Langmuir
JT  - Langmuir : the ACS journal of surfaces and colloids
JID - 9882736
RN  - 0 (Recombinant Proteins)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
RN  - PVI5M0M1GW (Filgrastim)
SB  - IM
MH  - Adsorption
MH  - Circular Dichroism
MH  - Filgrastim
MH  - Granulocyte Colony-Stimulating Factor/*chemistry
MH  - Recombinant Proteins/chemistry
MH  - Spectrometry, Mass, Secondary Ion
MH  - Surface Properties
MH  - Temperature
EDAT- 2013/11/28 06:00
MHDA- 2015/04/16 06:00
CRDT- 2013/11/27 06:00
PHST- 2013/11/27 06:00 [entrez]
PHST- 2013/11/28 06:00 [pubmed]
PHST- 2015/04/16 06:00 [medline]
AID - 10.1021/la403607m [doi]
PST - ppublish
SO  - Langmuir. 2013 Dec 17;29(50):15573-8. doi: 10.1021/la403607m. Epub 2013 Dec 4.