PMID- 24275061
OWN - NLM
STAT- MEDLINE
DCOM- 20140227
LR  - 20211021
IS  - 1872-8421 (Electronic)
IS  - 0165-5728 (Print)
IS  - 0165-5728 (Linking)
VI  - 266
IP  - 1-2
DP  - 2014 Jan 15
TI  - IFNgamma-stimulated dendritic cell exosomes as a potential therapeutic for 
      remyelination.
PG  - 12-23
LID - S0165-5728(13)00309-3 [pii]
LID - 10.1016/j.jneuroim.2013.10.014 [doi]
AB  - Dendritic cells (DCs) release exosomes with different characteristics based on 
      stimulus. Here, we showed that DC cultures stimulated with low-level IFNgamma 
      released exosomes (IFNgamma-DC-Exos) that contained microRNA species that can 
      increase baseline myelination, reduce oxidative stress, and improve remyelination 
      following acute lysolecithin-induced demyelination. Furthermore, nasally 
      administered IFNgamma-DC-Exos increased CNS myelination in vivo. IFNgamma-DC-Exos were 
      preferentially taken up by oligodendrocytes, suggesting that they directly impact 
      oligodendrocytes to increase myelination. Thus, our results show great potential 
      for use of these IFNgamma-DC-Exos as a therapeutic to promote remyelination in 
      multiple sclerosis and dysmyelinating syndromes.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Pusic, Aya D
AU  - Pusic AD
AD  - Department of Neurology, The University of Chicago, Chicago, IL 60637, USA; 
      Committee on Neurobiology, The University of Chicago, Chicago, IL 60637, USA.
FAU - Pusic, Kae M
AU  - Pusic KM
AD  - Department of Neurology, The University of Chicago, Chicago, IL 60637, USA.
FAU - Clayton, Benjamin L L
AU  - Clayton BL
AD  - Department of Neurology, The University of Chicago, Chicago, IL 60637, USA; 
      Committee on Neurobiology, The University of Chicago, Chicago, IL 60637, USA.
FAU - Kraig, Richard P
AU  - Kraig RP
AD  - Department of Neurology, The University of Chicago, Chicago, IL 60637, USA; 
      Committee on Neurobiology, The University of Chicago, Chicago, IL 60637, USA. 
      Electronic address: rkraig@neurology.bsd.uchicago.edu.
LA  - eng
GR  - UL1 TR000430/TR/NCATS NIH HHS/United States
GR  - UL1TR000430/TR/NCATS NIH HHS/United States
GR  - R01 NS019108/NS/NINDS NIH HHS/United States
GR  - 5 PO1 HD 09402/HD/NICHD NIH HHS/United States
GR  - NS-19108/NS/NINDS NIH HHS/United States
GR  - T32 GM007839/GM/NIGMS NIH HHS/United States
GR  - 5T32GM007839-31/GM/NIGMS NIH HHS/United States
GR  - 1 UH 2 TR000918/TR/NCATS NIH HHS/United States
GR  - P01 HD009402/HD/NICHD NIH HHS/United States
GR  - UH2 TR000918/TR/NCATS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20131109
PL  - Netherlands
TA  - J Neuroimmunol
JT  - Journal of neuroimmunology
JID - 8109498
RN  - 0 (Interferon-alpha)
RN  - 0 (Lysophosphatidylcholines)
RN  - 0 (MicroRNAs)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Demyelinating Diseases/chemically induced/*drug therapy
MH  - Dendritic Cells/*drug effects/ultrastructure
MH  - Exosomes/*drug effects
MH  - Female
MH  - Glutathione/metabolism
MH  - Hippocampus/cytology/ultrastructure
MH  - In Vitro Techniques
MH  - Interferon-alpha/*pharmacology
MH  - Lysophosphatidylcholines/toxicity
MH  - Male
MH  - MicroRNAs/metabolism
MH  - Microglia/drug effects/metabolism/ultrastructure
MH  - Oligodendroglia/drug effects/metabolism
MH  - Oxidative Stress/drug effects
MH  - Pregnancy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rats, Wistar
MH  - Stem Cells/drug effects
PMC - PMC3920591
MID - NIHMS544085
OTO - NOTNLM
OT  - Demyelination
OT  - Dendritic cell
OT  - Exosomes
OT  - MicroRNA
OT  - Multiple sclerosis
OT  - Myelin
EDAT- 2013/11/28 06:00
MHDA- 2014/02/28 06:00
PMCR- 2015/01/15
CRDT- 2013/11/27 06:00
PHST- 2013/07/31 00:00 [received]
PHST- 2013/09/21 00:00 [revised]
PHST- 2013/10/30 00:00 [accepted]
PHST- 2013/11/27 06:00 [entrez]
PHST- 2013/11/28 06:00 [pubmed]
PHST- 2014/02/28 06:00 [medline]
PHST- 2015/01/15 00:00 [pmc-release]
AID - S0165-5728(13)00309-3 [pii]
AID - 10.1016/j.jneuroim.2013.10.014 [doi]
PST - ppublish
SO  - J Neuroimmunol. 2014 Jan 15;266(1-2):12-23. doi: 10.1016/j.jneuroim.2013.10.014. 
      Epub 2013 Nov 9.