PMID- 24278942
OWN - NLM
STAT- MEDLINE
DCOM- 20140430
LR  - 20181202
IS  - 1942-4434 (Electronic)
IS  - 0882-2786 (Linking)
VI  - 28
IP  - 6
DP  - 2013 Nov-Dec
TI  - In vitro and in vivo osteoinductive and osteoconductive properties of a synthetic 
      bone substitute.
PG  - e432-9
LID - 10.11607/jomi.te23 [doi]
AB  - PURPOSE: The present study tested a recently introduced bone substitute material 
      (BSM) with a novel structure to determine its osteoinductive and osteoconductive 
      properties in vitro and in vivo. The specific aims were to determine the 
      microstructure of the as-manufactured BSM, as analyzed with scanning electron 
      microscopy, and to characterize different cellular interactions. MATERIALS AND 
      METHODS: Human bone marrow stromal cells were cultured in the presence of the 
      BSM. In vitro, attachment of osteoblastlike cells (SAOS-2) to the BSM was 
      observed with the scanning electron microscope. The expression of genes related 
      to osteogenic differentiation (alkaline phosphatase, bone sialoprotein, type I 
      collagen, and osteocalcin) was determined by reverse-transcriptase polymerase 
      chain reaction. In vivo, bone formation was examined with a murine model of 
      ectopic bone formation through histology and computed tomographic scanning by 
      using tissue-engineered constructs with the BSM and ovine bone marrow stromal 
      cells. RESULTS: Early cellular attachment could be detected as early as 6 hours. 
      Cellular morphology developed in the following 66 hours toward a starlike 
      appearance. Human bone marrow stromal cells cultured in the presence of the BSM 
      showed no reduction in their viability. Osteocalcin was up-regulated during cell 
      culturing, demonstrating an osteoinductive effect of BSM. Histologic and computed 
      tomographic analyses showed the formation of new bone surrounding BSM particles, 
      and a vascular meshwork was observed in the porosity of the particles. 
      CONCLUSION: The analyzed bone substitute of synthetic origin presented 
      osteoinductive properties that may exert a differentiative stimulus upon 
      osteoprogenitor cells. The tested material allowed cellular adhesion of 
      osteoblastlike cells and, following tissue construct implantation in vivo, 
      supported the formation of new bone.
FAU - Conserva, Enrico
AU  - Conserva E
FAU - Foschi, Federico
AU  - Foschi F
FAU - Cancedda, Ranieri
AU  - Cancedda R
FAU - Mastrogiacomo, Maddalena
AU  - Mastrogiacomo M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Int J Oral Maxillofac Implants
JT  - The International journal of oral & maxillofacial implants
JID - 8611905
RN  - 0 (Bone Substitutes)
RN  - 0 (Collagen Type I)
RN  - 0 (Integrin-Binding Sialoprotein)
RN  - 104982-03-8 (Osteocalcin)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/genetics/metabolism
MH  - Animals
MH  - Bone Marrow Cells/*physiology
MH  - Bone Regeneration/*physiology
MH  - *Bone Substitutes
MH  - Bone and Bones/metabolism
MH  - Cell Adhesion/physiology
MH  - Cell Differentiation
MH  - Collagen Type I/genetics/metabolism
MH  - Gene Expression
MH  - Humans
MH  - Integrin-Binding Sialoprotein/genetics/metabolism
MH  - Mice
MH  - Microscopy, Electron, Scanning
MH  - Osteoblasts/physiology
MH  - Osteocalcin/genetics/metabolism
MH  - Porosity
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sheep
MH  - Stromal Cells/*physiology
MH  - Time Factors
MH  - Tissue Engineering/methods
EDAT- 2013/11/28 06:00
MHDA- 2014/05/03 06:00
CRDT- 2013/11/27 06:00
PHST- 2013/11/27 06:00 [entrez]
PHST- 2013/11/28 06:00 [pubmed]
PHST- 2014/05/03 06:00 [medline]
AID - 10.11607/jomi.te23 [doi]
PST - ppublish
SO  - Int J Oral Maxillofac Implants. 2013 Nov-Dec;28(6):e432-9. doi: 
      10.11607/jomi.te23.