PMID- 24280970
OWN - NLM
STAT- MEDLINE
DCOM- 20140915
LR  - 20181203
IS  - 1423-0313 (Electronic)
IS  - 0031-7012 (Linking)
VI  - 92
IP  - 5-6
DP  - 2013
TI  - Human umbilical cord-derived mesenchymal stem cells downregulate inflammatory 
      responses by shifting the Treg/Th17 profile in experimental colitis.
PG  - 257-64
LID - 10.1159/000354883 [doi]
AB  - BACKGROUND/AIMS: The aim of this study was to evaluate the effect and mechanisms 
      of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) on immune 
      responses in murine colitis. METHODS: Mice with dextran sulfate sodium 
      (DSS)-induced colitis were injected intraperitoneally with hUC-MSCs or human bone 
      marrow-derived MSCs. The cytokine levels from lamina propria mononuclear cells 
      (LPMCs) and colon tissue were measured using ELISA. Treg and Th17 cells were 
      analyzed using flow cytometry. The proliferation of LPMCs was assessed using Cell 
      Counting Kit-8. RESULTS: hUC-MSCs ameliorate DSS-induced colitis via the 
      downregulation of colon inflammatory responses. Furthermore, hUC-MSCs adjusted 
      modulation of Treg/Th17 cells in the spleen and mesenteric lymph nodes. hUC-MSCs 
      also inhibited LPMCs in vitro. CONCLUSION: hUC-MSCs may be an alternative source 
      of stem cells and are worthy of study in long-term clinical trials.
FAU - Li, Liren
AU  - Li L
AD  - Department of Gastroenterology, Affiliated Hospital of Nantong University, 
      Nantong, China.
FAU - Liu, Suzhe
AU  - Liu S
FAU - Xu, Yue
AU  - Xu Y
FAU - Zhang, Aixian
AU  - Zhang A
FAU - Jiang, Jinxia
AU  - Jiang J
FAU - Tan, Wei
AU  - Tan W
FAU - Xing, Jing
AU  - Xing J
FAU - Feng, Guijuan
AU  - Feng G
FAU - Liu, Hong
AU  - Liu H
FAU - Huo, Fangxin
AU  - Huo F
FAU - Tang, Qiyun
AU  - Tang Q
FAU - Gu, Zhifeng
AU  - Gu Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131120
PL  - Switzerland
TA  - Pharmacology
JT  - Pharmacology
JID - 0152016
RN  - 0 (Cytokines)
RN  - 9042-14-2 (Dextran Sulfate)
SB  - IM
MH  - Animals
MH  - Cell Proliferation
MH  - Colitis/immunology/pathology/*therapy
MH  - Cytokines/metabolism
MH  - Dextran Sulfate/toxicity
MH  - Disease Models, Animal
MH  - Down-Regulation
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Inflammation/immunology/therapy
MH  - Lymph Nodes/immunology
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mesenchymal Stem Cells/cytology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mucous Membrane/metabolism
MH  - Spleen/immunology
MH  - T-Lymphocytes, Regulatory/*immunology
MH  - Th17 Cells/*immunology
MH  - Umbilical Cord/cytology
EDAT- 2013/11/28 06:00
MHDA- 2014/09/16 06:00
CRDT- 2013/11/28 06:00
PHST- 2013/02/01 00:00 [received]
PHST- 2013/08/06 00:00 [accepted]
PHST- 2013/11/28 06:00 [entrez]
PHST- 2013/11/28 06:00 [pubmed]
PHST- 2014/09/16 06:00 [medline]
AID - 000354883 [pii]
AID - 10.1159/000354883 [doi]
PST - ppublish
SO  - Pharmacology. 2013;92(5-6):257-64. doi: 10.1159/000354883. Epub 2013 Nov 20.