PMID- 24281405
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20131127
LR  - 20211021
IS  - 1424-8247 (Print)
IS  - 1424-8247 (Electronic)
IS  - 1424-8247 (Linking)
VI  - 5
IP  - 4
DP  - 2012 Mar 28
TI  - Oral Administration of Shark Type II Collagen Suppresses Complete Freund's 
      Adjuvant-Induced Rheumatoid Arthritis in Rats.
PG  - 339-52
LID - 10.3390/ph5040339 [doi]
AB  - OBJECTIVE: Shark type II collagen (SCII) is extracted as a glycoprotein from the 
      cartilage of blue shark (Prionace glauca). We aim to confirm the effects of oral 
      tolerance of SCII on inflammatory and immune responses to the ankle joint of 
      rheumatoid-arthritis rats induced by Complete Freund's Adjuvant (CFA). MATERIALS 
      AND METHODS: The onset of rheumatoid arthritis (RA) was observed 14 +/- x days 
      after injection of CFA. Rats in the control group were treated with acetic acid 
      by oral administration (0.05 mmol kg-1d-1, days 14-28), while rats in 
      experimental groups were treated by oral administration with SCII (1 or 3 mg 
      kg-1d-1, days 14-28), Tripterygium wilfordii polyglycosidium (TWP) (10 mg 
      kg-1d-1, days 14-28), and bovine type II collagen from US (US-CII) (1 mg kg-1d-1, 
      days 14-28), respectively. The severity of arthritis was evaluated by the 
      articular swelling. The immunological indexes observed included delayed type 
      hypersensitivity (DTH) reaction, the level of interleukins 10 (IL-10) in rat 
      blood serum and morphological characterization. Mixed lymphocyte culture (MLC) 
      was performed to investigate the relationship between T cell apoptosis and 
      specific immune tolerance induced by SCII. RESULTS: Treatment with SCII for 2 
      weeks significantly attenuated the acute inflammation. The rats orally 
      administrated with SCII at the level of 3 mg kg-1d-1 (SCII 3) and US-CII had 
      decreased DTH reaction compared with rats in control group. Rats treated with 
      SCII 3 had the highest level of IL-10 with 102 pg/mL. SCII with concentration of 
      10 mug/L could help to significantly enhance level of Fas/Apo-1 in T cell in 
      vitro. The result of histological staining indicated that the recovery of the 
      articular membranes of ankle joint in SCII 3 group was greatly enhanced. 
      CONCLUSIONS: Our results suggest that appropriate dose of SCII can not only 
      ameliorate symptoms but also modify the disease process of 
      Complete-Freunds-Adjuvant-induced arthritis. Oral administration of SCII might be 
      a potential candidate as a novel drug for further investigation.
FAU - Chen, Lijuan
AU  - Chen L
AD  - Shanghai Ocean University, No. 999, Hu Cheng Loop-road, Lingang New City, 
      Shanghai 201306, China. whwu@shou.edu.cn.
FAU - Bao, Bin
AU  - Bao B
FAU - Wang, Nanping
AU  - Wang N
FAU - Xie, Jing
AU  - Xie J
FAU - Wu, Wenhui
AU  - Wu W
LA  - eng
PT  - Journal Article
DEP - 20120328
PL  - Switzerland
TA  - Pharmaceuticals (Basel)
JT  - Pharmaceuticals (Basel, Switzerland)
JID - 101238453
PMC - PMC3763645
EDAT- 2012/01/01 00:00
MHDA- 2012/01/01 00:01
PMCR- 2012/04/01
CRDT- 2013/11/28 06:00
PHST- 2011/11/07 00:00 [received]
PHST- 2012/02/12 00:00 [revised]
PHST- 2012/03/21 00:00 [accepted]
PHST- 2013/11/28 06:00 [entrez]
PHST- 2012/01/01 00:00 [pubmed]
PHST- 2012/01/01 00:01 [medline]
PHST- 2012/04/01 00:00 [pmc-release]
AID - ph5040339 [pii]
AID - pharmaceuticals-05-00339 [pii]
AID - 10.3390/ph5040339 [doi]
PST - epublish
SO  - Pharmaceuticals (Basel). 2012 Mar 28;5(4):339-52. doi: 10.3390/ph5040339.