PMID- 2428396
OWN - NLM
STAT- MEDLINE
DCOM- 19861107
LR  - 20190613
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 25
IP  - 15
DP  - 1986 Jul 29
TI  - Rotational dynamics of the Fc receptor for immunoglobulin E on 
      histamine-releasing rat basophilic leukemia cells.
PG  - 4397-401
AB  - The rotational diffusion of immunoglobulin E (IgE) bound to its specific Fc 
      receptor on the surface of living rat basophilic leukemia cells was determined 
      from time-resolved phosphorescence emission and anisotropy measurements. The 
      IgE-receptor complexes are mobile throughout the range of temperatures of 5-38 
      degrees C. The residual anisotropy does not reach zero, indicating that the 
      rotational diffusion is hindered. The values of rotational correlation times for 
      each temperature are consistent with dispersed receptors rotating freely in the 
      cell membrane and rule out any significant aggregation of occupied receptors 
      before cross-linking by antigen or anti-IgE antibodies. The rotational 
      correlation times decrease with increasing temperature from 65 microseconds at 
      5.5 degrees C to 23 microseconds at 38 degrees C. However, the degree of 
      orientational constraint experienced by the probe is unchanged. Thus, the 
      temperature dependence can be attributed primarily to a change in the effective 
      viscosity of the cellular plasma membrane. The phosphorescence depolarization 
      technique is very sensitive (our probe concentrations were 10-100 nM) and thus 
      generally applicable to studies of surface receptors and antigens on living 
      cells.
FAU - Zidovetzki, R
AU  - Zidovetzki R
FAU - Bartholdi, M
AU  - Bartholdi M
FAU - Arndt-Jovin, D
AU  - Arndt-Jovin D
FAU - Jovin, T M
AU  - Jovin TM
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Receptors, Fc)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Basophils/immunology/physiology
MH  - *Histamine Release
MH  - Immunoglobulin E/*metabolism
MH  - Kinetics
MH  - Leukemia, Experimental/*immunology/physiopathology
MH  - Luminescent Measurements
MH  - Rats
MH  - Receptors, Fc/*metabolism
MH  - Thermodynamics
EDAT- 1986/07/29 00:00
MHDA- 1986/07/29 00:01
CRDT- 1986/07/29 00:00
PHST- 1986/07/29 00:00 [pubmed]
PHST- 1986/07/29 00:01 [medline]
PHST- 1986/07/29 00:00 [entrez]
AID - 10.1021/bi00363a033 [doi]
PST - ppublish
SO  - Biochemistry. 1986 Jul 29;25(15):4397-401. doi: 10.1021/bi00363a033.