PMID- 24284395
OWN - NLM
STAT- MEDLINE
DCOM- 20140603
LR  - 20211021
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 14
IP  - 11
DP  - 2013 Nov 11
TI  - Combination BMSC and Niaspan treatment of stroke enhances white matter remodeling 
      and synaptic protein expression in diabetic rats.
PG  - 22221-32
LID - 10.3390/ijms141122221 [doi]
AB  - OBJECTIVE: White matter remodeling plays an important role in neurological 
      recovery after stroke. Bone marrow stromal cells (BMSCs) and Niaspan, an agent 
      which increases high density lipoprotein (HDL), each induces neurorestorative 
      effects and promotes white matter remodeling after stroke in non-diabetic rats. 
      In this study, we test whether combination of BMSCs with Niaspan induces an 
      enhanced white matter remodeling in the ischemic brain of diabetic rats. RESEARCH 
      DESIGN AND METHODS: Type-1 diabetes (T1DM) rats were subjected to transient 
      middle cerebral artery occlusion (MCAo) and treated with or without BMSCs; 
      Niaspan; and the combination of BMSCs + Niaspan daily for 14 days after MCAo. 
      Immunostaining for white matter remodeling and synaptic protein expression 
      including NG2; CNPase; BS (Bielschowsky silver); LFB (luxol fast blue); 
      Synaptophysin and SMI-31 immunostaining were performed. RESULTS: BMSC monotherapy 
      did not regulate NG2 and CNPase expression compared to T1DM control rats. Both, 
      combination of BMSCs + Niaspan treatment, and Niaspan monotherapy significantly 
      increase NG2 and CNPase expression compared to T1DM control. While combination 
      BMSC+Niaspan, BMSC monotherapy and Niaspan monotherapy groups all increase BS, 
      LFB, synaptophysin, and SMI-31 expression in the ischemic brain compared to 
      T1DM-MCAo control. In addition, the combination treatment significantly enhances 
      LFB, SMI-31, and Synaptophysin expression compared to BMSC monotherapy. 
      CONCLUSIONS: Combination treatment of stroke with BMSCs and Niaspan in T1DM rats 
      increases white matter remodeling and additively increases BMSC monotherapy 
      induced myelination and synaptic plasticity after stroke in T1DM rats.
FAU - Ye, Xinchun
AU  - Ye X
AD  - Department of Neurology, the Affiliated Hospital of Xuzhou Medical College, 
      Xuzhou 221002, China. jieli@neuro.hfh.edu.
FAU - Yan, Tao
AU  - Yan T
FAU - Chopp, Michael
AU  - Chopp M
FAU - Zacharek, Alex
AU  - Zacharek A
FAU - Ning, Ruizhuo
AU  - Ning R
FAU - Venkat, Poornima
AU  - Venkat P
FAU - Roberts, Cynthia
AU  - Roberts C
FAU - Chen, Jieli
AU  - Chen J
LA  - eng
GR  - R01 AG031811/AG/NIA NIH HHS/United States
GR  - R41 NS064708/NS/NINDS NIH HHS/United States
GR  - 1R41NS064708/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20131111
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Lipoproteins, HDL)
RN  - 2679MF687A (Niacin)
SB  - IM
MH  - Animals
MH  - Brain/drug effects/pathology/*ultrastructure
MH  - Diabetes Mellitus, Experimental/complications/metabolism
MH  - Diabetes Mellitus, Type 1/metabolism/pathology
MH  - Lipoproteins, HDL/biosynthesis
MH  - *Mesenchymal Stem Cells
MH  - Niacin/*administration & dosage
MH  - *Protein Biosynthesis
MH  - Rats
MH  - Stroke/*drug therapy/metabolism/pathology
MH  - Synapses/genetics/metabolism/ultrastructure
PMC - PMC3856061
EDAT- 2013/11/29 06:00
MHDA- 2014/06/04 06:00
PMCR- 2013/11/01
CRDT- 2013/11/29 06:00
PHST- 2013/08/01 00:00 [received]
PHST- 2013/10/16 00:00 [revised]
PHST- 2013/10/18 00:00 [accepted]
PHST- 2013/11/29 06:00 [entrez]
PHST- 2013/11/29 06:00 [pubmed]
PHST- 2014/06/04 06:00 [medline]
PHST- 2013/11/01 00:00 [pmc-release]
AID - ijms141122221 [pii]
AID - ijms-14-22221 [pii]
AID - 10.3390/ijms141122221 [doi]
PST - epublish
SO  - Int J Mol Sci. 2013 Nov 11;14(11):22221-32. doi: 10.3390/ijms141122221.