PMID- 24285391
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140623
LR  - 20131128
IS  - 1097-0231 (Electronic)
IS  - 0951-4198 (Linking)
VI  - 28
IP  - 1
DP  - 2014 Jan 15
TI  - Ultrahigh-performance liquid chromatography/electrospray ionization linear ion 
      trap Orbitrap mass spectrometry of antioxidants (amines and phenols) applied in 
      lubricant engineering.
PG  - 63-76
LID - 10.1002/rcm.6756 [doi]
AB  - RATIONALE: For the qualification and quantification of antioxidants (aromatic 
      amines and sterically hindered phenols), most of them applied as lubricant 
      additives, two ultrahigh-performance liquid chromatography (UHPLC) electrospray 
      ionization mass spectrometric methods applying the positive and negative ion mode 
      have been developed for lubricant design and engineering thus allowing e.g. the 
      study of the degradation of lubricants. METHODS: Based on the different chemical 
      properties of the two groups of antioxidants, two methods offering a fast 
      separation (10 min) without prior derivatization were developed. In order to 
      reach these requirements, UHPLC was coupled with an LTQ Orbitrap hybrid tandem 
      mass spectrometer with positive and negative ion electrospray ionization for 
      simultaneous detection of spectra from UHPLC-high-resolution (HR)-MS (full scan 
      mode) and UHPLC-low-resolution linear ion trap MS(2) (LITMS(2)), which we term 
      UHPLC/HRMS-LITMS(2). RESULTS: All 20 analytes investigated could be qualified by 
      an UHPLC/HRMS-LITMS(2) approach consisting of simultaneous UHPLC/HRMS (elemental 
      composition) and UHPLC/LITMS(2) (diagnostic product ions) according to EC 
      guidelines. Quantification was based on an UHPLC/LITMS(2) approach due to 
      increased sensitivity and selectivity compared to UHPLC/HRMS. Absolute 
      quantification was only feasible for seven analytes with well-specified purity of 
      references whereas relative quantification was obtainable for another nine 
      antioxidants. All of them showed good standard deviation and repeatability. 
      CONCLUSIONS: The combined methods allow qualitative and quantitative 
      determination of a wide variety of different antioxidants including 
      aminic/phenolic compounds applied in lubricant engineering. These data show that 
      the developed methods will be versatile tools for further research on 
      identification and characterization of the thermo-oxidative degradation products 
      of antioxidants in lubricants.
CI  - Copyright (c) 2013 John Wiley & Sons, Ltd.
FAU - Kassler, Alexander
AU  - Kassler A
AD  - Institute of Chemical Technologies and Analytics, Vienna University of 
      Technology, Vienna, Austria; Austrian Center of Competence for Tribology (AC2T 
      research GmbH), Wiener Neustadt, Austria.
FAU - Pittenauer, Ernst
AU  - Pittenauer E
FAU - Doerr, Nicole
AU  - Doerr N
FAU - Allmaier, Guenter
AU  - Allmaier G
LA  - eng
PT  - Journal Article
PL  - England
TA  - Rapid Commun Mass Spectrom
JT  - Rapid communications in mass spectrometry : RCM
JID - 8802365
EDAT- 2013/11/29 06:00
MHDA- 2013/11/29 06:01
CRDT- 2013/11/29 06:00
PHST- 2013/08/13 00:00 [received]
PHST- 2013/10/02 00:00 [revised]
PHST- 2013/10/03 00:00 [accepted]
PHST- 2013/11/29 06:00 [entrez]
PHST- 2013/11/29 06:00 [pubmed]
PHST- 2013/11/29 06:01 [medline]
AID - 10.1002/rcm.6756 [doi]
PST - ppublish
SO  - Rapid Commun Mass Spectrom. 2014 Jan 15;28(1):63-76. doi: 10.1002/rcm.6756.