PMID- 2428568
OWN - NLM
STAT- MEDLINE
DCOM- 19861113
LR  - 20171116
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 7
IP  - 5
DP  - 1986 Sep
TI  - The induction of the human T-cell growth factor receptor precedes the production 
      of RNA and occurs in the presence of inhibitors of RNA synthesis.
PG  - 453-62
AB  - The receptor for T-cell growth factor (TCGF) is an activation antigen that is 
      present in low amounts on a small fraction of resting T lymphocytes. The TCGF 
      receptor on human T cells can be detected with the anti-Tac monoclonal antibody 
      within 7-12 h of stimulating the cells with phytohemagglutinin (PHA). In the 
      current studies, we examined human lymphocytes cultured alone, with PHA, or with 
      PHA plus sufficient actinomycin-D to inhibit RNA synthesis. After varying 
      intervals, aliquots of the lymphocytes were stained with acridine orange (AO) or 
      pyronin-Y(PY) to measure RNA and/or with anti-Tac plus FITC goat anti-mouse Ig. 
      Tac expression began to increase after 6-8 h incubation with PHA, whereas 
      increases in PY or AO staining were not detected until 12 h or later. 
      Furthermore, the initial increase in Tac expression was not affected by 
      sufficient actinomycin-D to block all detectable nucleic acid synthesis. 
      Therefore, it appears that the initial expression of TCGF receptors detected 
      after lymphocyte activation does not require de novo production of RNA.
FAU - Redelman, D
AU  - Redelman D
FAU - Wormsley, S
AU  - Wormsley S
LA  - eng
GR  - CA 38396/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Surface)
RN  - 0 (Phytohemagglutinins)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Interleukin-2)
RN  - 0 (Sesquiterpenes)
RN  - 0 (Tumor Necrosis Factor Receptor Superfamily, Member 7)
RN  - 1CC1JFE158 (Dactinomycin)
RN  - 63231-63-0 (RNA)
RN  - 71117-22-1 (K 76 carboxylic acid)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Antigens, Surface/metabolism
MH  - Cells, Cultured
MH  - DNA/analysis/metabolism
MH  - Dactinomycin/*pharmacology
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Phytohemagglutinins/pharmacology
MH  - RNA/analysis/*metabolism
MH  - Receptors, Immunologic/*metabolism
MH  - Receptors, Interleukin-2
MH  - Sesquiterpenes/pharmacology
MH  - T-Lymphocytes/*metabolism
MH  - Time Factors
MH  - Transcription, Genetic/drug effects
MH  - Tumor Necrosis Factor Receptor Superfamily, Member 7
EDAT- 1986/09/01 00:00
MHDA- 1986/09/01 00:01
CRDT- 1986/09/01 00:00
PHST- 1986/09/01 00:00 [pubmed]
PHST- 1986/09/01 00:01 [medline]
PHST- 1986/09/01 00:00 [entrez]
AID - 10.1002/cyto.990070511 [doi]
PST - ppublish
SO  - Cytometry. 1986 Sep;7(5):453-62. doi: 10.1002/cyto.990070511.