PMID- 24286327
OWN - NLM
STAT- MEDLINE
DCOM- 20150204
LR  - 20211021
IS  - 1976-670X (Electronic)
IS  - 1976-6696 (Print)
IS  - 1976-6696 (Linking)
VI  - 47
IP  - 6
DP  - 2014 Jun
TI  - Inhibitory effects of oroxylin A on endothelial protein C receptor shedding in 
      vitro and in vivo.
PG  - 336-41
AB  - Endothelial cell protein C receptor (EPCR) plays important roles in blood 
      coagulation and inflammation. EPCR activity is markedly changed by ectodomain 
      cleavage and release as the soluble EPCR. EPCR can be shed from the cell surface, 
      which is mediated by tumor necrosis factor-alpha converting enzyme (TACE). Oroxylin A 
      (OroA), a major component of Scutellaria baicalensis Georgi, is known to exhibit 
      anti-angiogenic, antiinflammation, and anti-invasive activities. However, little 
      is known about the effects of OroA on EPCR shedding. Data showed that OroA 
      induced potent inhibition of phorbol-12-myristate 13-acetate (PMA), tumor 
      necrosis factor (TNF)-alpha, interleukin (IL)-1beta and on cecal ligation and puncture 
      (CLP)-induced EPCR shedding through suppression of TACE expression and activity. 
      In addition, treatment with OroA resulted in reduced PMA-stimulated 
      phosphorylation of p38, extracellular regulated kinases (ERK) 1/2, and c-Jun 
      N-terminal kinase (JNK). These results demonstrate the potential of OroA as an 
      anti-sEPCR shedding reagent against PMA and CLP-mediated EPCR shedding.
FAU - Ku, Sae-Kwang
AU  - Ku SK
AD  - Department of Anatomy and Histology, College of Oriental Medicine, Daegu Haany 
      University, Gyeongsan 712-715, Korea.
FAU - Han, Min-Su
AU  - Han MS
AD  - Laboratory for Arthritis and Bone Biology, Fatima Research Institute, Daegu 
      Fatima Hospital, Daegu 701-724, Korea.
FAU - Lee, Min Young
AU  - Lee MY
AD  - College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, 
      Kyungpook National University, Daegu 702-701, Korea.
FAU - Lee, You-Mie
AU  - Lee YM
AD  - College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, 
      Kyungpook National University, Daegu 702-701, Korea.
FAU - Bae, Jong-Sup
AU  - Bae JS
AD  - College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, 
      Kyungpook National University, Daegu 702-701, Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - BMB Rep
JT  - BMB reports
JID - 101465334
RN  - 0 (Blood Coagulation Factors)
RN  - 0 (Flavonoids)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (activated protein C receptor)
RN  - 53K24Z586G (5,7-dihydroxy-6-methoxy-2-phenylchromen-4-one)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 3.4.24.- (ADAM Proteins)
RN  - EC 3.4.24.86 (ADAM17 Protein)
RN  - EC 3.4.24.86 (ADAM17 protein, human)
RN  - EC 3.4.24.86 (Adam17 protein, mouse)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - ADAM Proteins/metabolism
MH  - ADAM17 Protein
MH  - Animals
MH  - Blood Coagulation Factors/*metabolism
MH  - Disease Models, Animal
MH  - Down-Regulation/*drug effects
MH  - Flavonoids/chemistry/*pharmacology/therapeutic use
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - Interleukin-1beta/pharmacology
MH  - JNK Mitogen-Activated Protein Kinases/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mitogen-Activated Protein Kinase 1/metabolism
MH  - Mitogen-Activated Protein Kinase 3/metabolism
MH  - Phosphorylation/drug effects
MH  - Receptors, Cell Surface/*metabolism
MH  - Scutellaria baicalensis/chemistry/metabolism
MH  - Sepsis/drug therapy/etiology/metabolism
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
PMC - PMC4163868
EDAT- 2013/11/30 06:00
MHDA- 2015/02/05 06:00
PMCR- 2014/07/01
CRDT- 2013/11/30 06:00
PHST- 2013/09/02 00:00 [received]
PHST- 2013/11/30 06:00 [entrez]
PHST- 2013/11/30 06:00 [pubmed]
PHST- 2015/02/05 06:00 [medline]
PHST- 2014/07/01 00:00 [pmc-release]
AID - 2499 [pii]
AID - BMB-47-336 [pii]
AID - 10.5483/bmbrep.2014.47.6.198 [doi]
PST - ppublish
SO  - BMB Rep. 2014 Jun;47(6):336-41. doi: 10.5483/bmbrep.2014.47.6.198.