PMID- 24287159
OWN - NLM
STAT- MEDLINE
DCOM- 20140526
LR  - 20140127
IS  - 1618-0429 (Electronic)
IS  - 0932-4739 (Linking)
VI  - 50
IP  - 1
DP  - 2014 Feb
TI  - Identification of the pathogenic ciliate Pseudocohnilembus persalinus 
      (Oligohymenophorea: Scuticociliatia) by fluorescence in situ hybridization.
PG  - 16-24
LID - S0932-4739(13)00067-9 [pii]
LID - 10.1016/j.ejop.2013.09.004 [doi]
AB  - Many scuticociliatid ciliates are regarded as devastating pathogens in 
      aquaculture. Among these, Pseudocohnilembus persalinus is a facultative pathogen 
      that often results in refractory diseases of mariculture fish. Although 
      traditional silver staining methods have been successfully used to identify these 
      ciliates, their identification is hampered by their small size and their 
      morphological similarity to closely related species. We designed an alternative 
      method of identification of P. persalinus using an SSU-rDNA targeted 
      oligonucleotide probe labeled with a fluorochrome, and optimized in a 
      fluorescence in situ hybridization (FISH) protocol. The assay results in a clear 
      identification by strong fluorescence signals from the oligonucleotide probe. The 
      method can be used for quick and early detection of P. persalinus infections on 
      host fish, as well as other susceptible organisms in aquiculture water. It may 
      also be used in studies of the geographical distribution of this scuticociliate.
CI  - Copyright (c) 2013 Elsevier GmbH. All rights reserved.
FAU - Zhan, Zifeng
AU  - Zhan Z
AD  - Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; 
      Department of Ecology, University of Kaiserslautern, Kaiserslautern 67653, 
      Germany.
FAU - Stoeck, Thorsten
AU  - Stoeck T
AD  - Department of Ecology, University of Kaiserslautern, Kaiserslautern 67653, 
      Germany.
FAU - Dunthorn, Micah
AU  - Dunthorn M
AD  - Department of Ecology, University of Kaiserslautern, Kaiserslautern 67653, 
      Germany.
FAU - Xu, Kuidong
AU  - Xu K
AD  - Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. 
      Electronic address: kxu@qdio.ac.cn.
LA  - eng
SI  - GENBANK/JQ956554
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131022
PL  - Germany
TA  - Eur J Protistol
JT  - European journal of protistology
JID - 8917383
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - Animals
MH  - Ciliophora Infections/diagnosis/*veterinary
MH  - DNA, Ribosomal/genetics
MH  - Fish Diseases/*diagnosis
MH  - Fisheries/*methods
MH  - Fluorescent Dyes/chemistry
MH  - In Situ Hybridization, Fluorescence/*veterinary
MH  - Molecular Sequence Data
MH  - Oligohymenophorea/classification/cytology/*genetics
MH  - Oligonucleotide Probes/genetics
MH  - Phylogeny
OTO - NOTNLM
OT  - Fluorescence in situ hybridization
OT  - Molecular methods
OT  - Oligonucleotide probes
OT  - SSU-rDNA
OT  - Scuticociliates
EDAT- 2013/11/30 06:00
MHDA- 2014/05/27 06:00
CRDT- 2013/11/30 06:00
PHST- 2013/07/24 00:00 [received]
PHST- 2013/08/31 00:00 [revised]
PHST- 2013/09/02 00:00 [accepted]
PHST- 2013/11/30 06:00 [entrez]
PHST- 2013/11/30 06:00 [pubmed]
PHST- 2014/05/27 06:00 [medline]
AID - S0932-4739(13)00067-9 [pii]
AID - 10.1016/j.ejop.2013.09.004 [doi]
PST - ppublish
SO  - Eur J Protistol. 2014 Feb;50(1):16-24. doi: 10.1016/j.ejop.2013.09.004. Epub 2013 
      Oct 22.