PMID- 24287823
OWN - NLM
STAT- MEDLINE
DCOM- 20150615
LR  - 20211021
IS  - 1573-2576 (Electronic)
IS  - 0360-3997 (Linking)
VI  - 37
IP  - 3
DP  - 2014 Jun
TI  - Effect of IL-15 and natural killer cells on osteoclasts and osteoblasts in a 
      mouse coculture.
PG  - 657-69
LID - 10.1007/s10753-013-9782-0 [doi]
AB  - This study analyzes the effect of interleukin-15 (IL-15) on osteoclast formation 
      using a coculture of mouse osteoblasts and bone marrow cells (BMCs) stimulated 
      with prostaglandin E2 (PGE2), which both have important role in rheumatoid 
      arthritis (RA) and periodontal disease (PD). BMCs isolate lacking T (BM(T-)) or 
      NK (BM(NK-)) cells, BMCs with no cells removed (BM(T+NK+)), purified NK cells, 
      and purified T cells were each cocultured with osteoblasts in the presence or 
      absence of PGE2 and/or IL-15. The number of both osteoclasts and osteoblasts was 
      decreased by IL-15 in a dose-dependent manner in BM(T+NK+), BM(T-). However, the 
      reductions were improved in BM(NK-). The expression of caspase3 in osteoblasts 
      cocultured with NK cells was increased in a dose-dependent manner by IL-15. IL-15 
      stimulates apoptosis of osteoblasts via activation of NK cells. Since osteoblasts 
      have an important role in bone formation, IL-15 may be an inflammatory bone 
      destructive factor in RA and PD.
FAU - Takeda, Hiroaki
AU  - Takeda H
AD  - Department of Periodontology, School of Dentistry, Aichi Gakuin University, 2-11 
      Suemoridori, Chikusa-ku, Nagoya, Aichi, 464-8651, Japan.
FAU - Kikuchi, Takeshi
AU  - Kikuchi T
FAU - Soboku, Kyoko
AU  - Soboku K
FAU - Okabe, Iichiro
AU  - Okabe I
FAU - Mizutani, Hiroki
AU  - Mizutani H
FAU - Mitani, Akio
AU  - Mitani A
FAU - Ishihara, Yuichi
AU  - Ishihara Y
FAU - Noguchi, Toshihide
AU  - Noguchi T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Inflammation
JT  - Inflammation
JID - 7600105
RN  - 0 (Interleukin-15)
RN  - EC 3.4.22.- (Caspase 3)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Animals
MH  - Apoptosis/immunology
MH  - Arthritis, Rheumatoid/immunology
MH  - Bone Development/immunology
MH  - Bone Marrow Cells/immunology
MH  - Bone and Bones/immunology
MH  - Caspase 3/biosynthesis
MH  - Cell Differentiation/drug effects/*immunology
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Dinoprostone/*pharmacology
MH  - Interleukin-15/*pharmacology
MH  - Killer Cells, Natural/immunology
MH  - Mice
MH  - Osteoblasts/*cytology
MH  - Osteoclasts/*cytology
MH  - Periodontal Diseases/immunology
MH  - T-Lymphocytes/immunology
EDAT- 2013/11/30 06:00
MHDA- 2015/06/16 06:00
CRDT- 2013/11/30 06:00
PHST- 2013/11/30 06:00 [entrez]
PHST- 2013/11/30 06:00 [pubmed]
PHST- 2015/06/16 06:00 [medline]
AID - 10.1007/s10753-013-9782-0 [doi]
PST - ppublish
SO  - Inflammation. 2014 Jun;37(3):657-69. doi: 10.1007/s10753-013-9782-0.