PMID- 2428931
OWN - NLM
STAT- MEDLINE
DCOM- 19861107
LR  - 20190908
IS  - 0162-0134 (Print)
IS  - 0162-0134 (Linking)
VI  - 27
IP  - 3
DP  - 1986 Jul
TI  - Enhancement of in vitro ribonucleic acid synthesis on chromium(III)-bound 
      chromatin.
PG  - 179-89
AB  - Z chromatin-chromium (Cr) complex, prepared from mouse liver chromatin and CrCl3, 
      showed a significantly enhanced template activity for in vitro RNA synthesis. 
      Digestion experiments with this complex using micrococcal nuclease and DNase I 
      suggested that Cr(III) preferentially binds to linker regions rather than core 
      regions of chromatin. Further, it was found that Cr(III) binds to DNA and 
      nonhistone proteins (NHP), but hardly to histones. Moreover, the template 
      activity of an NHP-Cr complex, when added to a DNA-histones complex, was 
      inhibited remarkably. The template activity of the chromatin-Cr complex was not 
      significantly altered by proteinase K digestion. Furthermore, experiments using 
      rifampicin and [gamma-32P]guanosine 5'-triphosphate (GTP) demonstrated an 
      increase in the number of initiation sites in the chromatin-Cr complex. These 
      results suggest that, in this in vitro system, Cr(III) preferentially binds to 
      DNA in chromatin and causes an increase in the number of initiation sites, thus 
      enhancing RNA synthesis.
FAU - Ohba, H
AU  - Ohba H
FAU - Suketa, Y
AU  - Suketa Y
FAU - Okada, S
AU  - Okada S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Inorg Biochem
JT  - Journal of inorganic biochemistry
JID - 7905788
RN  - 0 (Chromatin)
RN  - 0 (Indicators and Reagents)
RN  - 0R0008Q3JB (Chromium)
RN  - 63231-63-0 (RNA)
RN  - VJT6J7R4TR (Rifampin)
SB  - IM
MH  - Animals
MH  - Chromatin/drug effects/*metabolism
MH  - Chromium/*pharmacology
MH  - Indicators and Reagents
MH  - Kinetics
MH  - Liver/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - RNA/*biosynthesis
MH  - Rifampin/pharmacology
MH  - Templates, Genetic
MH  - Transcription, Genetic/*drug effects
EDAT- 1986/07/01 00:00
MHDA- 1986/07/01 00:01
CRDT- 1986/07/01 00:00
PHST- 1986/07/01 00:00 [pubmed]
PHST- 1986/07/01 00:01 [medline]
PHST- 1986/07/01 00:00 [entrez]
AID - 0162-0134(86)80059-9 [pii]
AID - 10.1016/0162-0134(86)80059-9 [doi]
PST - ppublish
SO  - J Inorg Biochem. 1986 Jul;27(3):179-89. doi: 10.1016/0162-0134(86)80059-9.