PMID- 24291325
OWN - NLM
STAT- MEDLINE
DCOM- 20150219
LR  - 20181202
IS  - 1090-2139 (Electronic)
IS  - 0889-1591 (Linking)
VI  - 39
DP  - 2014 Jul
TI  - The effects of moderate exercise on chronic stress-induced intestinal barrier 
      dysfunction and antimicrobial defense.
PG  - 99-106
LID - S0889-1591(13)00544-8 [pii]
LID - 10.1016/j.bbi.2013.11.013 [doi]
AB  - The purpose of this study was to examine the effect of moderate exercise on 
      repeated restraint stress (RRS)-induced intestinal barrier dysfunction and 
      explore possible mechanisms in a mouse model. Male Balb/c mice (6weeks) were 
      randomized into 7 groups: CON functioned as controls with no intervention; RRS 
      was subjected to 6h per day RRS for 7 consecutive days; RRS+SWIM received 30min 
      per day of swimming prior to RRS; CON+SWIM only received 30min per day of 
      swimming; and the other groups received one session of 30min swimming prior to 
      sacrifice at 1-, 3- and 6h recovery. Intestinal permeability was quantified with 
      FITC-dextran. Bacterial translocation was determined by quantification of 
      bacterial colony forming units (CFUs) in cultured mesenteric lymph nodes (MLN), 
      and with fluorescence in situ hybridization (FISH). Antimicrobial related gene 
      expression at baseline and 1h after one session of 30min swimming was tested by 
      quantitative real-time polymerase chain reaction (Q-PCR) in small intestinal 
      segments. Protein expression of 5 genes with statistically significant increase 
      was measured at baseline, and 1-, 3- and 6h post-swimming using enzyme-linked 
      immunosorbent assay (ELISA). Thirty minutes per day of swimming before RRS 
      attenuated bacterial translocations and maintained intestinal permeability. Gene 
      expression and protein levels for four antimicrobial peptides (alpha-defensin 5, 
      beta-defensin 1, RegIIIbeta and RegIIIgamma) were significantly increased after one 30min 
      swimming session. In conclusion, moderate exercise attenuated chronic 
      stress-induced intestinal barrier dysfunction in mice, possibly due to 
      augmentation of antimicrobial responses in the small intestine.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Luo, Beibei
AU  - Luo B
AD  - Key Laboratory of Exercise and Health Sciences of Ministry of Education, Shanghai 
      University of Sport, Shanghai, China.
FAU - Xiang, Dao
AU  - Xiang D
AD  - Department of Diving Medicine, Naval Medical Research Institute, Shanghai, China; 
      Department of Cell Biology, Second Military Medical University, Shanghai, China.
FAU - Nieman, David C
AU  - Nieman DC
AD  - Human Performance Laboratory, Appalachian State University, North Carolina 
      Research Campus, Kannapolis, NC, United States.
FAU - Chen, Peijie
AU  - Chen P
AD  - Key Laboratory of Exercise and Health Sciences of Ministry of Education, Shanghai 
      University of Sport, Shanghai, China. Electronic address: chenpeijie@sus.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131127
PL  - Netherlands
TA  - Brain Behav Immun
JT  - Brain, behavior, and immunity
JID - 8800478
RN  - 0 (Antimicrobial Cationic Peptides)
SB  - IM
MH  - Animals
MH  - Antimicrobial Cationic Peptides/*genetics
MH  - Bacterial Physiological Phenomena
MH  - Gene Expression
MH  - Intestinal Mucosa/*metabolism
MH  - Intestines/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Permeability
MH  - Physical Conditioning, Animal/*physiology
MH  - Restraint, Physical
MH  - Stress, Psychological/*metabolism
MH  - Swimming/physiology
OTO - NOTNLM
OT  - Antimicrobial peptides
OT  - Chronic stress
OT  - Intestinal barrier
OT  - Moderate exercise
OT  - Mucosal immunity
OT  - Small intestine
OT  - Swimming
EDAT- 2013/12/03 06:00
MHDA- 2015/02/20 06:00
CRDT- 2013/12/03 06:00
PHST- 2013/07/31 00:00 [received]
PHST- 2013/10/17 00:00 [revised]
PHST- 2013/11/18 00:00 [accepted]
PHST- 2013/12/03 06:00 [entrez]
PHST- 2013/12/03 06:00 [pubmed]
PHST- 2015/02/20 06:00 [medline]
AID - S0889-1591(13)00544-8 [pii]
AID - 10.1016/j.bbi.2013.11.013 [doi]
PST - ppublish
SO  - Brain Behav Immun. 2014 Jul;39:99-106. doi: 10.1016/j.bbi.2013.11.013. Epub 2013 
      Nov 27.