PMID- 24291717
OWN - NLM
STAT- MEDLINE
DCOM- 20140715
LR  - 20131230
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 944
DP  - 2014 Jan 1
TI  - Determination of sec-O-glucosylhamaudol in rat plasma by gradient elution liquid 
      chromatography-mass spectrometry.
PG  - 35-8
LID - S1570-0232(13)00614-4 [pii]
LID - 10.1016/j.jchromb.2013.11.001 [doi]
AB  - Sec-O-glucosylhamaudol is one of the major bioactive compounds of the 
      Saposhnikoviae Radix. A simple and selective liquid chromatography-mass 
      spectrometry (LC-MS) method for determination of sec-O-glucosylhamaudol in rat 
      plasma was developed. After addition of carbamazepine as internal standard (IS), 
      protein precipitation with acetonitrile-methanol (9:1, v/v) was used as sample 
      preparation. Chromatographic separation was achieved on a Zorbax SB-C18 
      (2.1mmx150mm, 5mum) column with acetonitrile-0.1% formic acid in water as mobile 
      phase with gradient elution. Electrospray ionization (ESI) source was applied and 
      operated in positive ion mode; selective ion monitoring (SIM) mode was used for 
      quantification using target fragment ions m/z 439 for sec-O-glucosylhamaudol and 
      m/z 237 for the IS. Calibration plots were linear over the range of 50-8000ng/mL 
      for sec-O-glucosylhamaudol in rat plasma. Mean recovery of sec-O-glucosylhamaudol 
      in plasma was in the range of 74.8-83.7%. Intra-day and inter-day precision were 
      both <15%. This method was successfully applied in pharmacokinetic study after 
      intravenous administration of 2.5mg/kg sec-O-glucosylhamaudol in rats.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Wen, Congcong
AU  - Wen C
AD  - Laboratory Animal Centre of Wenzhou Medical University, Wenzhou 325035, China.
FAU - Lin, Chongliang
AU  - Lin C
AD  - The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, 
      China.
FAU - Cai, Xiaojun
AU  - Cai X
AD  - Analytical and Testing Center of Wenzhou Medical University, Wenzhou 325035, 
      China.
FAU - Ma, Jianshe
AU  - Ma J
AD  - Analytical and Testing Center of Wenzhou Medical University, Wenzhou 325035, 
      China.
FAU - Wang, Xianqin
AU  - Wang X
AD  - Analytical and Testing Center of Wenzhou Medical University, Wenzhou 325035, 
      China. Electronic address: lankywang@foxmail.com.
LA  - eng
PT  - Journal Article
DEP - 20131115
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Chromones)
RN  - 33CM23913M (Carbamazepine)
SB  - IM
MH  - Animals
MH  - Carbamazepine
MH  - Chromatography, Liquid/*methods
MH  - Chromones/*blood/chemistry/pharmacokinetics
MH  - Linear Models
MH  - Male
MH  - Mass Spectrometry/*methods
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
OTO - NOTNLM
OT  - LC-MS
OT  - Pharmacokinetics
OT  - Rat plasma
OT  - Sec-O-glucosylhamaudol
EDAT- 2013/12/03 06:00
MHDA- 2014/07/16 06:00
CRDT- 2013/12/03 06:00
PHST- 2013/09/07 00:00 [received]
PHST- 2013/10/30 00:00 [revised]
PHST- 2013/11/01 00:00 [accepted]
PHST- 2013/12/03 06:00 [entrez]
PHST- 2013/12/03 06:00 [pubmed]
PHST- 2014/07/16 06:00 [medline]
AID - S1570-0232(13)00614-4 [pii]
AID - 10.1016/j.jchromb.2013.11.001 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Jan 1;944:35-8. doi: 
      10.1016/j.jchromb.2013.11.001. Epub 2013 Nov 15.