PMID- 24291740
OWN - NLM
STAT- MEDLINE
DCOM- 20140820
LR  - 20191210
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 196
DP  - 2014 Feb
TI  - The development of a loop-mediated isothermal amplification assay for rapid and 
      sensitive detection of abalone herpesvirus DNA.
PG  - 199-203
LID - S0166-0934(13)00482-5 [pii]
LID - 10.1016/j.jviromet.2013.11.011 [doi]
AB  - A loop-mediated isothermal amplification (LAMP) assay was developed for the 
      detection of abalone herpesvirus DNA. Two pairs of primers were designed, based 
      on the sequence of the DNA polymerase gene of abalone herpesvirus. The reaction 
      temperature and time were optimized to 63 degrees C and 60min, respectively. LAMP 
      amplicons were analyzed by 2% agarose gel electrophoresis or by visual inspection 
      of a colour change emitted by fluorescent dye. The method developed was specific 
      for the detection of abalone herpesvirus, without cross-reactions with other 
      tested herpesviruses including ostreid herpesvirus 1 (OsHV-1), European eel 
      herpesvirus, koi herpesvirus (KHV) and an avian herpesvirus. The LAMP assay was 
      100 folds more sensitive than a conventional PCR and 10 folds less sensitive than 
      a SYBR Green PCR. These results indicate that the developed LAMP assay is a 
      simple, rapid, sensitive, specific and reliable technique for the detection of 
      abalone herpesvirus.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Chen, M H
AU  - Chen MH
AD  - Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan 
      University, Taipei, Taiwan; Tzu Chi College of Technology, Hualien, Taiwan.
FAU - Kuo, S T
AU  - Kuo ST
AD  - National Institute for Animal Health, Tansui, Taiwan.
FAU - Renault, T
AU  - Renault T
AD  - Ifremer, Unite Sante Genetique et Microbiologie des Mollusques, Laboratoire de 
      Genetique et Pathologie des Mollusques Marins, 17390 La Tremblade, France.
FAU - Chang, P H
AU  - Chang PH
AD  - Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan 
      University, Taipei, Taiwan. Electronic address: penheng@ntu.edu.tw.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131127
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - DNA Primers/genetics
MH  - DNA, Viral/genetics/*isolation & purification
MH  - Gastropoda/*virology
MH  - Herpesviridae/genetics/*isolation & purification
MH  - Nucleic Acid Amplification Techniques/*methods
MH  - Sensitivity and Specificity
MH  - Temperature
OTO - NOTNLM
OT  - Abalone
OT  - Haliotis diversicolor supertexta
OT  - Herpesvirus
OT  - Loop-mediated isothermal amplification
OT  - Polymerase chain reaction
OT  - SYBR green PCR
EDAT- 2013/12/03 06:00
MHDA- 2014/08/21 06:00
CRDT- 2013/12/03 06:00
PHST- 2013/07/17 00:00 [received]
PHST- 2013/11/16 00:00 [revised]
PHST- 2013/11/20 00:00 [accepted]
PHST- 2013/12/03 06:00 [entrez]
PHST- 2013/12/03 06:00 [pubmed]
PHST- 2014/08/21 06:00 [medline]
AID - S0166-0934(13)00482-5 [pii]
AID - 10.1016/j.jviromet.2013.11.011 [doi]
PST - ppublish
SO  - J Virol Methods. 2014 Feb;196:199-203. doi: 10.1016/j.jviromet.2013.11.011. Epub 
      2013 Nov 27.