PMID- 24292461 OWN - NLM STAT- MEDLINE DCOM- 20140305 LR - 20191210 IS - 0273-1223 (Print) IS - 0273-1223 (Linking) VI - 68 IP - 10 DP - 2013 TI - Start-up of a granular sludge sequencing batch reactor for the treatment of 2,4-dichlorophenol-contaminated wastewater. PG - 2151-7 LID - 10.2166/wst.2013.470 [doi] AB - In this study, a granular sludge sequencing batch reactor (GSBR) was started-up for the biological aerobic treatment of wastewater containing highly toxic 2,4-dichlorophenol (2,4-DCP), in presence of readily biodegradable sodium acetate (NaAc) as the growth substrate. Different influent concentrations of NaAc (420-800 mg/L) and 2,4-DCP (0-20 mg/L), as well as different operating conditions (i.e. cycle length), were tested in order to determine the optimal strategy for successful GSBR start-up: stable granulation and complete 2,4-DCP removal were achieved only when high NaAc influent concentration and volumetric organic loading rates (800 mg/L and 1.9 kgCOD/(m(3).d), respectively), prolonged reaction phase (cycle time of 4 hours) and gradual increase of 2,4-DCP concentration in the influent were applied, thus providing useful information for process optimization in view of future scale-up. Granules were initially colonized by fungi which progressively disappeared during the start-up process, and complete 2,4-DCP removal was mostly due to bacterial activity, in particular Betaproteobacteria, as shown by fluorescence in situ hybridization (FISH). FAU - Milia, S AU - Milia S AD - Institute of Environmental Geology and Geoengineering (IGAG-CNR), Piazza d'Armi, 09123 Cagliari, Italy E-mail: smilia@unica.it. FAU - Porcu, R AU - Porcu R FAU - Rossetti, S AU - Rossetti S FAU - Carucci, A AU - Carucci A LA - eng PT - Evaluation Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - Water Sci Technol JT - Water science and technology : a journal of the International Association on Water Pollution Research JID - 9879497 RN - 0 (Chlorophenols) RN - R669TG1950 (2,4-dichlorophenol) SB - IM MH - Bacteria/*metabolism MH - *Bioreactors MH - Chlorophenols/*metabolism MH - Water Purification/*instrumentation EDAT- 2013/12/03 06:00 MHDA- 2014/03/07 06:00 CRDT- 2013/12/03 06:00 PHST- 2013/12/03 06:00 [entrez] PHST- 2013/12/03 06:00 [pubmed] PHST- 2014/03/07 06:00 [medline] AID - 10.2166/wst.2013.470 [doi] PST - ppublish SO - Water Sci Technol. 2013;68(10):2151-7. doi: 10.2166/wst.2013.470.