PMID- 24292580
OWN - NLM
STAT- MEDLINE
DCOM- 20140716
LR  - 20131202
IS  - 1791-7549 (Electronic)
IS  - 0258-851X (Linking)
VI  - 27
IP  - 6
DP  - 2013 Nov-Dec
TI  - Distribution changes of epithelial dendritic cells in canine cornea and mucous 
      membranes related to hematopoietic stem cell transplantation.
PG  - 761-71
AB  - BACKGROUND/AIM: Dendritic cells (DCs) are important immune mediators following 
      allogeneic hematopoietic stem cell transplantation (HSCT). We screened for DC 
      frequency in the cornea and oral mucous membranes after HSCT by confocal laser 
      scanning microscopy (CLSM) in a canine model. MATERIALS AND METHODS: In vivo CLSM 
      images of the epithelia were taken the day before and on days 28, 56 and 112 
      following HSCT. Peripheral blood counts and chimerism were determined. RESULTS: 
      An increase of DCs after HSCT was detected in each animal in both investigated 
      tissue types. Highest DC numbers in the flew and the gingiva were detected on day 
      28 and in the corneal epithelium on day 56 after HSCT, respectively. CONCLUSION: 
      Changes of DCs in ocular and non-ocular mucous membranes can be monitored by CLSM 
      in vivo. The DC frequency in the cornea and mucosa changes following HSCT. DC 
      recovery is rapid and their numbers correlate with the development of chimerism 
      of peripheral blood mononuclear cells.
FAU - Killian, Doreen
AU  - Killian D
AD  - University of Rostock, Division of Medicine, Department of Hematology, Oncology 
      and Palliative Medicine, Ernst-Heydemann-Str. 6, 18057 Rostock, Germany. 
      christian.junghanss@med.uni-rostock.de.
FAU - Reichard, Maria
AU  - Reichard M
FAU - Knueppel, Anne
AU  - Knueppel A
FAU - Lange, Sandra
AU  - Lange S
FAU - Sekora, Anett
AU  - Sekora A
FAU - Knuebel, Gudrun
AU  - Knuebel G
FAU - Murua Escobar, Hugo
AU  - Murua Escobar H
FAU - Guthoff, Rudolf
AU  - Guthoff R
FAU - Stachs, Oliver
AU  - Stachs O
FAU - Junghanss, Christian
AU  - Junghanss C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - In Vivo
JT  - In vivo (Athens, Greece)
JID - 8806809
SB  - IM
MH  - Animals
MH  - Cell Count
MH  - Dendritic Cells/*immunology
MH  - Dogs
MH  - Epithelium, Corneal/*cytology
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Microscopy, Confocal
MH  - Mouth Mucosa/*cytology
MH  - Organ Specificity
OTO - NOTNLM
OT  - Dendritic cells
OT  - chimerism
OT  - confocal laser scanning microscopy
OT  - epithelium
OT  - stem cell transplantation
EDAT- 2013/12/03 06:00
MHDA- 2014/07/17 06:00
CRDT- 2013/12/03 06:00
PHST- 2013/12/03 06:00 [entrez]
PHST- 2013/12/03 06:00 [pubmed]
PHST- 2014/07/17 06:00 [medline]
AID - 27/6/761 [pii]
PST - ppublish
SO  - In Vivo. 2013 Nov-Dec;27(6):761-71.