PMID- 24295333
OWN - NLM
STAT- MEDLINE
DCOM- 20141114
LR  - 20140207
IS  - 1535-3907 (Electronic)
IS  - 1535-3893 (Linking)
VI  - 13
IP  - 2
DP  - 2014 Feb 7
TI  - Protein secretome of moss plants (Physcomitrella patens) with emphasis on changes 
      induced by a fungal elicitor.
PG  - 447-59
LID - 10.1021/pr400827a [doi]
AB  - Studies on extracellular proteins (ECPs) contribute to understanding of the 
      multifunctional nature of apoplast. Unlike vascular plants (tracheophytes), 
      little information about ECPs is available from nonvascular plants, such as 
      mosses (bryophytes). In this study, moss plants (Physcomitrella patens) were 
      grown in liquid culture and treated with chitosan, a water-soluble form of chitin 
      that occurs in cell walls of fungi and insects and elicits pathogen defense in 
      plants. ECPs released to the culture medium were compared between 
      chitosan-treated and nontreated control cultures using quantitative mass 
      spectrometry (Orbitrap) and 2-DE-LC-MS/MS. Over 400 secreted proteins were 
      detected, of which 70% were homologous to ECPs reported in tracheophyte 
      secretomes. Bioinformatics analyses using SignalP and SecretomeP predicted 
      classical signal peptides for secretion (37%) or leaderless secretion (27%) for 
      most ECPs of P. patens, but secretion of the remaining proteins (36%) could not 
      be predicted using bioinformatics. Cultures treated with chitosan contained 72 
      proteins not found in untreated controls, whereas 27 proteins found in controls 
      were not detected in chitosan-treated cultures. Pathogen defense-related proteins 
      dominated in the secretome of P. patens, as reported in tracheophytes. These 
      results advance knowledge on protein secretomes of plants by providing a 
      comprehensive account of ECPs of a bryophyte.
FAU - Lehtonen, Mikko T
AU  - Lehtonen MT
AD  - Department of Agricultural Sciences, University of Helsinki , PO Box 27, FI-00014 
      Helsinki, Finland.
FAU - Takikawa, Yoshihiro
AU  - Takikawa Y
FAU - Ronnholm, Gunilla
AU  - Ronnholm G
FAU - Akita, Motomu
AU  - Akita M
FAU - Kalkkinen, Nisse
AU  - Kalkkinen N
FAU - Ahola-Iivarinen, Elina
AU  - Ahola-Iivarinen E
FAU - Somervuo, Panu
AU  - Somervuo P
FAU - Varjosalo, Markku
AU  - Varjosalo M
FAU - Valkonen, Jari P T
AU  - Valkonen JP
LA  - eng
GR  - R01 GM61992/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20131206
PL  - United States
TA  - J Proteome Res
JT  - Journal of proteome research
JID - 101128775
RN  - 0 (Plant Proteins)
RN  - 0 (Proteome)
SB  - IM
MH  - Bryopsida/*metabolism/microbiology
MH  - Chromatography, Liquid
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Fungi/*physiology
MH  - Oligonucleotide Array Sequence Analysis
MH  - Plant Proteins/genetics/*metabolism
MH  - *Proteome
MH  - Tandem Mass Spectrometry
EDAT- 2013/12/04 06:00
MHDA- 2014/11/15 06:00
CRDT- 2013/12/04 06:00
PHST- 2013/12/04 06:00 [entrez]
PHST- 2013/12/04 06:00 [pubmed]
PHST- 2014/11/15 06:00 [medline]
AID - 10.1021/pr400827a [doi]
PST - ppublish
SO  - J Proteome Res. 2014 Feb 7;13(2):447-59. doi: 10.1021/pr400827a. Epub 2013 Dec 6.