PMID- 24295678
OWN - NLM
STAT- MEDLINE
DCOM- 20140721
LR  - 20191210
IS  - 1873-7072 (Electronic)
IS  - 0308-8146 (Linking)
VI  - 149
DP  - 2014 Apr 15
TI  - Determination of residual enrofloxacin in food samples by a sensitive method of 
      chemiluminescence enzyme immunoassay.
PG  - 71-5
LID - S0308-8146(13)01458-1 [pii]
LID - 10.1016/j.foodchem.2013.10.024 [doi]
AB  - A chemiluminescence enzyme immunoassay (CLEIA) based on the HRP-luminol-H(2)O(2) 
      chemiluminescence system for highly sensitive detection of enrofloxacin (ENR) was 
      proposed in this study. Key factors that affect the precision and accuracy for 
      the determination of ENR residues were optimised. Under the optimal conditions, 
      the proposed method showed an excellent performance. The linearity range for 
      method developed for determination of ENR was 0.35-1.0 ng/mL with a correlation 
      coefficient greater than 0.994. The limit of detection was 0.03 ng/mL and the 
      relative standard deviations (RSDs) were less than 9.4% and 13.0% for intra-day 
      and inter-day assays. The proposed method was satisfactorily applied to determine 
      ENR in milk, eggs, and honey samples at three spiked levels (0.4, 0.7, and 1.0 
      ng/mL) and the recoveries ranged from 92.4% to 104.2% for milk, 93.8% to 103.2% 
      for eggs and 94.1% to 105.0% for honey, respectively. Compared the results of 
      CLEIA with those of ELISA and HPLC, the advantages of the CLEIA were further 
      confirmed. Moreover, one 96-well microtiter plate coated with anti-ENR can be 
      used to detect multiple samples at the same time, which indicated that the CLEIA 
      using HRP-luminol-H(2)O(2) system was a sensitive, high throughput and real-time 
      method for ENR residues analysis.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Yu, Fei
AU  - Yu F
AD  - College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
FAU - Yu, Songcheng
AU  - Yu S
FAU - Yu, Lanlan
AU  - Yu L
FAU - Li, Yanqiang
AU  - Li Y
FAU - Wu, Yongjun
AU  - Wu Y
FAU - Zhang, Hongquan
AU  - Zhang H
FAU - Qu, Lingbo
AU  - Qu L
FAU - Harrington, Peter de B
AU  - Harrington Pde B
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131026
PL  - England
TA  - Food Chem
JT  - Food chemistry
JID - 7702639
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Fluoroquinolones)
RN  - 3DX3XEK1BN (Enrofloxacin)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*analysis
MH  - Eggs/*analysis
MH  - Enrofloxacin
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Fluoroquinolones/*analysis
MH  - Food Contamination/*analysis
MH  - Honey/*analysis
MH  - Limit of Detection
MH  - Luminescent Measurements/*methods
MH  - Milk/*chemistry
OTO - NOTNLM
OT  - Chemiluminescence enzyme immunoassay (CLEIA)
OT  - Enrofloxacin (ENR)
OT  - Enzyme-linked immunosorbent assay (ELISA)
OT  - High-performance liquid chromatography (HPLC)
EDAT- 2013/12/04 06:00
MHDA- 2014/07/22 06:00
CRDT- 2013/12/04 06:00
PHST- 2012/12/28 00:00 [received]
PHST- 2013/09/11 00:00 [revised]
PHST- 2013/10/06 00:00 [accepted]
PHST- 2013/12/04 06:00 [entrez]
PHST- 2013/12/04 06:00 [pubmed]
PHST- 2014/07/22 06:00 [medline]
AID - S0308-8146(13)01458-1 [pii]
AID - 10.1016/j.foodchem.2013.10.024 [doi]
PST - ppublish
SO  - Food Chem. 2014 Apr 15;149:71-5. doi: 10.1016/j.foodchem.2013.10.024. Epub 2013 
      Oct 26.