PMID- 24297308
OWN - NLM
STAT- MEDLINE
DCOM- 20140725
LR  - 20211021
IS  - 1559-131X (Electronic)
IS  - 1357-0560 (Linking)
VI  - 31
IP  - 1
DP  - 2014 Jan
TI  - MicroRNA-205 suppresses proliferation and promotes apoptosis in laryngeal 
      squamous cell carcinoma.
PG  - 785
LID - 10.1007/s12032-013-0785-3 [doi]
AB  - MicroRNAs were reported to be involved in the modulation of tumor development. 
      The aim of our study was to investigate the effect of miR-205 on proliferation 
      and apoptosis of laryngeal squamous cell carcinoma (LSCC) and seek associations 
      between miR-205 and Bcl-2 using in vitro and in vivo methods. Real-time qPCR was 
      used to analyze the expression of miR-205 in LSCC samples and Hep-2 cell line. 
      Apoptosis, cell cycle, and proliferation (MTT) assays were performed to test the 
      apoptosis and proliferation of LSCC cells after miR-205 transfection. Bcl-2 
      expression in cells was assessed with Western blotting. The tumorigenicity of 
      LSCC cells was evaluated in nude mice model. MiR-205 was significantly 
      down-regulated in LSCC tissues compared to adjacent normal tissues. Lower 
      expression of miR-205 was indicated to be statistically related with advanced 
      clinical stage and T3-4 grades. We found that restoration of miR-205 
      down-regulated the proliferative markers of dihydrofolate reductase and 
      proliferating cell nuclear antigen and apoptotic regulator of Bcl-2. The findings 
      in vitro and in vivo showed miR-205 could suppress cell proliferation and induce 
      cell apoptosis. In addition, Bcl-2 was identified as one of the direct targets of 
      miR-205 in LSCC cells. These results suggest that miR-205 may play as a tumor 
      suppressor in LSCC, probably by targeting Bcl-2 and serve as a potential target 
      for therapeutic intervention.
FAU - Tian, Linli
AU  - Tian L
AD  - Department of Otolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, Harbin, 150081, China.
FAU - Zhang, Jiarui
AU  - Zhang J
FAU - Ge, Jingchun
AU  - Ge J
FAU - Xiao, Hui
AU  - Xiao H
FAU - Lu, Jianguang
AU  - Lu J
FAU - Fu, Songbin
AU  - Fu S
FAU - Liu, Ming
AU  - Liu M
FAU - Sun, Yanan
AU  - Sun Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131203
PL  - United States
TA  - Med Oncol
JT  - Medical oncology (Northwood, London, England)
JID - 9435512
RN  - 0 (MIRN205 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
SB  - IM
MH  - Aged
MH  - Animals
MH  - *Apoptosis
MH  - Carcinoma, Squamous Cell/*genetics/*pathology
MH  - Cell Cycle
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Head and Neck Neoplasms/*genetics/*pathology
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/*pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - Neoplasm Transplantation
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
MH  - Squamous Cell Carcinoma of Head and Neck
EDAT- 2013/12/04 06:00
MHDA- 2014/07/26 06:00
CRDT- 2013/12/04 06:00
PHST- 2013/10/15 00:00 [received]
PHST- 2013/11/22 00:00 [accepted]
PHST- 2013/12/04 06:00 [entrez]
PHST- 2013/12/04 06:00 [pubmed]
PHST- 2014/07/26 06:00 [medline]
AID - 10.1007/s12032-013-0785-3 [doi]
PST - ppublish
SO  - Med Oncol. 2014 Jan;31(1):785. doi: 10.1007/s12032-013-0785-3. Epub 2013 Dec 3.