PMID- 24298991
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140806
LR  - 20131227
IS  - 1520-5207 (Electronic)
IS  - 1520-5207 (Linking)
VI  - 117
IP  - 51
DP  - 2013 Dec 27
TI  - Stimulated emission properties of fluorophores by CW-STED single molecule 
      spectroscopy.
PG  - 16405-15
LID - 10.1021/jp409330t [doi]
AB  - Fluorophores useful for STimulated Emission Depletion (STED) spectroscopy must 
      fulfill strict requirements on depletion efficiency and photostability. These 
      parameters determine the effective resolution of STED imaging. Resolution is 
      typically measured on 30-80 nm spheres heavily decorated with STED bright 
      fluorophores, limiting the possibility to estimate the true resolution achievable 
      on a specific dye. Here we show how single molecule STED microscopy provides an 
      estimate of the fluorophore stimulated emission cross section and of its 
      photostability under STED irradiation. Fluorescein, a green and a yellow mutant 
      of GFP, are tested, and the results are discussed and compared to those obtained 
      with Chromeo488-covered 80 nm spheres on a commercial continuous-wave STED 
      microscope.
FAU - Bouzin, Margaux
AU  - Bouzin M
AD  - Physics Department, Universita degli Studi di Milano-Bicocca , Piazza della 
      Scienza 3, 20126, Milano, Italy.
FAU - Chirico, Giuseppe
AU  - Chirico G
FAU - D'Alfonso, Laura
AU  - D'Alfonso L
FAU - Sironi, Laura
AU  - Sironi L
FAU - Soavi, Giancarlo
AU  - Soavi G
FAU - Cerullo, Giulio
AU  - Cerullo G
FAU - Campanini, Barbara
AU  - Campanini B
FAU - Collini, Maddalena
AU  - Collini M
LA  - eng
PT  - Journal Article
DEP - 20131212
PL  - United States
TA  - J Phys Chem B
JT  - The journal of physical chemistry. B
JID - 101157530
EDAT- 2013/12/05 06:00
MHDA- 2013/12/05 06:01
CRDT- 2013/12/05 06:00
PHST- 2013/12/05 06:00 [entrez]
PHST- 2013/12/05 06:00 [pubmed]
PHST- 2013/12/05 06:01 [medline]
AID - 10.1021/jp409330t [doi]
PST - ppublish
SO  - J Phys Chem B. 2013 Dec 27;117(51):16405-15. doi: 10.1021/jp409330t. Epub 2013 
      Dec 12.