PMID- 24302477
OWN - NLM
STAT- MEDLINE
DCOM- 20140915
LR  - 20151119
IS  - 1098-2795 (Electronic)
IS  - 1040-452X (Linking)
VI  - 81
IP  - 1
DP  - 2014 Jan
TI  - Palmitoyl-protein thioesterase 1 (PPT1): an obesity-induced rat testicular marker 
      of reduced fertility.
PG  - 55-65
LID - 10.1002/mrd.22281 [doi]
AB  - Male obesity may lead to declines in testosterone levels, reproductive hormonal 
      profile, and semen quantity. To assess the effects of obesity on spermatogenesis, 
      Sprague-Dawley rats fed a high-fat diet served as a model of induced obesity. The 
      litter sizes for females mated to obese males were significantly lower as 
      compared to females mated with normal-diet-fed controls. Their serum high-density 
      lipoprotein, low-density lipoprotein, cholesterol, and estradiol levels increased 
      in obese males, but testosterone and follicle-stimulating hormone levels 
      decreased. Testicular morphology disruptions included Sertoli-cell atrophy, 
      disrupted tight junctions, and mitochondrial degeneration in spermatogenic cells. 
      To further investigate the molecular mechanisms leading to high-fat-diet-induced 
      changes, we employed testicular proteomic analysis on rats fed both types of 
      diet. Three spots were up-regulated in rats fed a high-fat diet whereas two 
      others were downregulated. One of the upregulated spots was palmitoyl-protein 
      thioesterase 1 (PPT1), a lipoprotein metabolizing related enzyme localized to 
      Sertoli cells. In a Sertoli-cell line cultured in a high-fat supplemented medium, 
      PPT1 abundance was accompanied by increases in the endocytic vesicle-associated 
      protein, clathrin, and decreases in the tight junctional proteins, ZO-1 and 
      occludin. In conclusion, declines in rat male fertility induced by a high-fat 
      diet are associated with an altered testicular protein expression pattern as well 
      as disruption of testicular Sertoli-cell and spermatogenic-cell morphology. PPT1 
      expression may provide a testicular marker of reduced fertility in obese males, 
      as increases in its expression may be detrimental to Sertoli-cell function during 
      spermatogenesis.
CI  - (c) 2013 Wiley Periodicals, Inc.
FAU - Liu, Yue
AU  - Liu Y
AD  - Department of Human Anatomy, Histology and Embryology, Shanghai Key Laboratory 
      for Reproductive Medicine, School of Medicine, Shanghai Jiao Tong University, 
      Shanghai, 200025, China.
FAU - Zhao, Wenzhen
AU  - Zhao W
FAU - Gu, Guobao
AU  - Gu G
FAU - Lu, Liming
AU  - Lu L
FAU - Feng, Jingsheng
AU  - Feng J
FAU - Guo, Qiangsu
AU  - Guo Q
FAU - Ding, Zhide
AU  - Ding Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131203
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 0 (Biomarkers)
RN  - 0 (DNA Primers)
RN  - 0 (Lipoproteins)
RN  - 0 (Occludin)
RN  - 0 (Proteome)
RN  - 0 (Tjp1 protein, rat)
RN  - 0 (Zonula Occludens-1 Protein)
RN  - 3XMK78S47O (Testosterone)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
RN  - EC 3.1.2.- (Thiolester Hydrolases)
RN  - EC 3.1.2.22 (palmitoyl-protein thioesterase)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Biomarkers/*metabolism
MH  - Body Weight
MH  - DNA Primers/genetics
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Fluorescent Antibody Technique
MH  - Follicle Stimulating Hormone/blood
MH  - Gene Expression Regulation/*physiology
MH  - Infertility, Male/etiology/*physiopathology
MH  - Lipoproteins/blood
MH  - Litter Size/physiology
MH  - Male
MH  - Mass Spectrometry
MH  - Obesity/complications/*physiopathology
MH  - Occludin/metabolism
MH  - Proteome/metabolism
MH  - Proteomics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Real-Time Polymerase Chain Reaction
MH  - Sertoli Cells/physiology
MH  - Spermatogenesis/*physiology
MH  - Testis/metabolism/*pathology
MH  - Testosterone/blood
MH  - Thiolester Hydrolases/*metabolism
MH  - Zonula Occludens-1 Protein/metabolism
EDAT- 2013/12/05 06:00
MHDA- 2014/09/16 06:00
CRDT- 2013/12/05 06:00
PHST- 2013/06/12 00:00 [received]
PHST- 2013/11/05 00:00 [accepted]
PHST- 2013/12/05 06:00 [entrez]
PHST- 2013/12/05 06:00 [pubmed]
PHST- 2014/09/16 06:00 [medline]
AID - 10.1002/mrd.22281 [doi]
PST - ppublish
SO  - Mol Reprod Dev. 2014 Jan;81(1):55-65. doi: 10.1002/mrd.22281. Epub 2013 Dec 3.