PMID- 24302835
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20131204
LR  - 20211021
IS  - 0976-4879 (Print)
IS  - 0975-7406 (Electronic)
IS  - 0975-7406 (Linking)
VI  - 5
IP  - 4
DP  - 2013 Oct
TI  - Mitigation of starch-induced postprandial glycemic spikes in rats by 
      antioxidants-rich extract of Cicer arietinum Linn. seeds and sprouts.
PG  - 270-6
LID - 10.4103/0975-7406.120077 [doi]
AB  - INTRODUCTION: Consumption of highly processed calories dense diet leads abrupt 
      increase in postprandial blood glucose level, which in turn induces immediate 
      oxidative stress. Postprandial hyperglycemia (PPHG) and resultant oxidative 
      stress is one of the earliest detectable abnormalities in diabetes prone 
      individuals, independent risk factor for development of cardiovascular disorders 
      (CVD), a major pathophysiological link between diabetes and CVD and an important 
      contributing factor in atherogenesis even in non-diabetic individuals. Therefore, 
      dietary supplements mitigating PPHG spikes along with potent antioxidant 
      activities may help decrease development of PPHG and oxidative stress induced 
      pathogenesis. OBJECTIVES: The study evaluated free radicals scavenging, 
      antioxidant properties and intestinal alpha-glucosidase inhibitory activity in 
      methanol extract of two varieties of Cicer arietinum Linn viz. Bengal gram and 
      Kabuli chana and green gram (Vigna radiata Linn. Wilczek) raw grains and their 
      sprouts and studied their influence on starch-induced postprandial glycemic 
      excursion in rats. MATERIALS AND METHODS: Healthy grains were procured from local 
      markets. Free radicals scavenging antioxidant and glucose-induced hemoglobin 
      (Hb)-glycation inhibition activities were analyzed using standard in vitro 
      procedures. In vitro antihyperglycemic activity was evaluated by assessing rat 
      intestinal alpha-glucosidase inhibitory activity. Influence on starch-induced 
      postprandial glycemic excursion in rats was studied by pre-treatment of rats with 
      extracts. RESULTS: Compared with raw seeds increase in total polyphenol and 
      flavonoids concentration in green gram sprouts and Kabuli chana sprouts (KCs) 
      were observed. Total protein concentrations in sprouts did not differ from 
      non-sprouted grains. 2,2'- Azinobis (3-ethyl benzthiazoline-6-sulphonic acid) 
      cation scavenging activity was more than twice in Bengal gram sprouts of (BGs) 
      and KCs than their raw seeds. 2,2-diphenyl-1-picrylhydrazyl, hydrogen peroxide 
      scavenging, nitro blue tetrazolium reducing and glucose-induced Hb-glycation 
      inhibitory activity did not differ from non-sprouted raw grains. Increase in rat 
      intestinal alpha-glucosidase inhibitory activity was observed in BGs and KCs. BGs 
      significantly mitigated 1(st) 30 min starch-induced postprandial glycemic 
      excursions and reduced 2 h postprandial glycemic load. CONCLUSION: Sprouting 
      leads dynamic changes in free radicals scavenging potentials and antioxidant 
      activities in grains. Consumption of seeds as well as BGs before the starch-rich 
      meal can significantly mitigate 1(st) 30 min postprandial glycemic excursion and 
      reduce 2 h postprandial glycemic burden.
FAU - Tiwari, Ashok Kumar
AU  - Tiwari AK
AD  - Medicinal Chemistry and Pharmacology Division, Council of Scientific and 
      Industrial Research-Indian Institute of Chemical Technology, Hyderabad, Andhra 
      Pradesh, India.
FAU - Sahana, Chinthapatla
AU  - Sahana C
FAU - Zehra, Amtul
AU  - Zehra A
FAU - Madhusudana, Kuncha
AU  - Madhusudana K
FAU - Kumar, Domati Anand
AU  - Kumar DA
FAU - Agawane, Sachin Bharat
AU  - Agawane SB
LA  - eng
PT  - Journal Article
PL  - India
TA  - J Pharm Bioallied Sci
JT  - Journal of pharmacy & bioallied sciences
JID - 101537209
PMC - PMC3831740
OTO - NOTNLM
OT  - Antioxidant activity
OT  - Bengal gram sprouts
OT  - oxidative stress
OT  - postprandial glycemic excursion
OT  - postprandial hyperglycemia
COIS- Conflict of Interest: None declared.
EDAT- 2013/12/05 06:00
MHDA- 2013/12/05 06:01
PMCR- 2013/10/01
CRDT- 2013/12/05 06:00
PHST- 2013/01/18 00:00 [received]
PHST- 2013/07/10 00:00 [revised]
PHST- 2013/08/05 00:00 [accepted]
PHST- 2013/12/05 06:00 [entrez]
PHST- 2013/12/05 06:00 [pubmed]
PHST- 2013/12/05 06:01 [medline]
PHST- 2013/10/01 00:00 [pmc-release]
AID - JPBS-5-270 [pii]
AID - 10.4103/0975-7406.120077 [doi]
PST - ppublish
SO  - J Pharm Bioallied Sci. 2013 Oct;5(4):270-6. doi: 10.4103/0975-7406.120077.