PMID- 24304973
OWN - NLM
STAT- MEDLINE
DCOM- 20141118
LR  - 20211021
IS  - 1476-5470 (Electronic)
IS  - 1466-4879 (Print)
IS  - 1466-4879 (Linking)
VI  - 15
IP  - 2
DP  - 2014 Mar
TI  - Follicular lymphoma-protective HLA class II variants correlate with increased 
      HLA-DQB1 protein expression.
PG  - 133-6
LID - 10.1038/gene.2013.64 [doi]
AB  - Multiple follicular lymphoma (FL) susceptibility single-nucleotide polymorphisms 
      in the human leukocyte antigen (HLA) class I and II regions have been identified, 
      including rs6457327, rs3117222, rs2647012, rs10484561, rs9268853 and rs2621416. 
      Here we validated previous expression quantitative trait loci results with 
      real-time reverse transcription quantitative PCR and investigated protein 
      expression in B-lymphoblastoid cell lines and primary dendritic cells using flow 
      cytometry, cell-based enzyme-linked immunosorbent assay and western blotting. We 
      confirmed that FL-protective rs2647012-linked variants, in high linkage 
      disequilibrium with the extended haplotype DRB1*15:01-DQA1*01:02-DQB1*06:02, 
      correlate with increased HLA-DQB1 expression. This association remained 
      significant at the protein level and was reproducible across different cell 
      types. We also found that differences in HLA-DQB1 expression were not related to 
      changes in activation markers or class II, major histocompatibility complex, 
      transactivator expression, suggesting the role of an alternative regulatory 
      mechanism. However, functional analysis using RegulomeDB did not reveal any 
      relevant regulatory candidates. Future studies should focus on the clinical 
      relevance of increased HLA-DQB1 protein expression facilitating tumor cell 
      removal through increased immune surveillance.
FAU - Sille, F C M
AU  - Sille FC
AD  - Division of Environmental Health Sciences, School of Public Health, University of 
      California, Berkeley, CA, USA.
FAU - Conde, L
AU  - Conde L
AD  - Department of Epidemiology, Comprehensive Cancer Center, University of Alabama, 
      Birmingham, AL, USA.
FAU - Zhang, J
AU  - Zhang J
AD  - Department of Epidemiology, Comprehensive Cancer Center, University of Alabama, 
      Birmingham, AL, USA.
FAU - Akers, N K
AU  - Akers NK
AD  - Division of Environmental Health Sciences, School of Public Health, University of 
      California, Berkeley, CA, USA.
FAU - Sanchez, S
AU  - Sanchez S
AD  - Division of Environmental Health Sciences, School of Public Health, University of 
      California, Berkeley, CA, USA.
FAU - Maltbaek, J
AU  - Maltbaek J
AD  - Division of Environmental Health Sciences, School of Public Health, University of 
      California, Berkeley, CA, USA.
FAU - Riby, J E
AU  - Riby JE
AD  - Department of Epidemiology, Comprehensive Cancer Center, University of Alabama, 
      Birmingham, AL, USA.
FAU - Smith, M T
AU  - Smith MT
AD  - Division of Environmental Health Sciences, School of Public Health, University of 
      California, Berkeley, CA, USA.
FAU - Skibola, C F
AU  - Skibola CF
AD  - Department of Epidemiology, Comprehensive Cancer Center, University of Alabama, 
      Birmingham, AL, USA.
LA  - eng
GR  - R01 CA104682/CA/NCI NIH HHS/United States
GR  - R01 CA154643/CA/NCI NIH HHS/United States
GR  - CA104682/CA/NCI NIH HHS/United States
GR  - CA154643/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20131205
PL  - England
TA  - Genes Immun
JT  - Genes and immunity
JID - 100953417
RN  - 0 (HLA-DQ beta-Chains)
RN  - 0 (HLA-DQB1 antigen)
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Cells, Cultured
MH  - Dendritic Cells/immunology
MH  - Gene Frequency
MH  - Genetic Predisposition to Disease
MH  - HLA-DQ beta-Chains/*biosynthesis/*genetics/immunology
MH  - Haplotypes/genetics/immunology
MH  - Humans
MH  - Linkage Disequilibrium/genetics
MH  - Lipopolysaccharides
MH  - Lymphocyte Activation
MH  - Lymphoma, Follicular/*genetics/immunology
MH  - Polymorphism, Single Nucleotide
MH  - Quantitative Trait Loci/immunology
PMC - PMC6279234
MID - NIHMS547241
COIS- CONFLICT OF INTEREST The authors declare no conflict of interest.
EDAT- 2013/12/07 06:00
MHDA- 2014/11/19 06:00
PMCR- 2018/12/04
CRDT- 2013/12/06 06:00
PHST- 2013/08/28 00:00 [received]
PHST- 2013/10/30 00:00 [revised]
PHST- 2013/10/30 00:00 [accepted]
PHST- 2013/12/06 06:00 [entrez]
PHST- 2013/12/07 06:00 [pubmed]
PHST- 2014/11/19 06:00 [medline]
PHST- 2018/12/04 00:00 [pmc-release]
AID - gene201364 [pii]
AID - 10.1038/gene.2013.64 [doi]
PST - ppublish
SO  - Genes Immun. 2014 Mar;15(2):133-6. doi: 10.1038/gene.2013.64. Epub 2013 Dec 5.