PMID- 24305555
OWN - NLM
STAT- MEDLINE
DCOM- 20140929
LR  - 20131219
IS  - 1463-9084 (Electronic)
IS  - 1463-9076 (Linking)
VI  - 16
IP  - 4
DP  - 2014 Jan 28
TI  - Adsorption of NGF and BDNF derived peptides on gold surfaces.
PG  - 1536-44
LID - 10.1039/c3cp52499j [doi]
AB  - This study tackles the interaction between gold surfaces and two peptide 
      fragments named NGF(1-14) and BDNF(1-12), able to mimic the proliferative 
      activity of the nerve growth factor (NGF) and the brain derived neurotrophic 
      factor (BDNF), respectively. The physical adsorption processes on the solid 
      surface from both single and binary peptide solutions, at physiological and acid 
      pH, were investigated by QCM-D and CD experiments, as well as by molecular 
      dynamics calculations. The relevant physicochemical properties at the hybrid 
      bio-interface, including peptide-surface interaction, conformational changes, 
      hydrodynamic thickness, viscoelastic parameters, and competitive vs. synergic 
      behaviour of the two peptide fragments towards the surface, were scrutinized. 
      Biological assays with neuronal cells pointed to the maintenance of the 
      biological activity of NGF(1-14) and BDNF(1-12) peptide molecules within the 
      adlayers on the gold surface.
FAU - Forte, Giuseppe
AU  - Forte G
AD  - Department of Pharmaceutical Sciences, University of Catania, Viale Andrea Doria, 
      6, I-95125, Italy.
FAU - Travaglia, Alessio
AU  - Travaglia A
FAU - Magri, Antonio
AU  - Magri A
FAU - Satriano, Cristina
AU  - Satriano C
FAU - La Mendola, Diego
AU  - La Mendola D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Phys Chem Chem Phys
JT  - Physical chemistry chemical physics : PCCP
JID - 100888160
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Peptides)
RN  - 7440-57-5 (Gold)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Adsorption
MH  - Brain-Derived Neurotrophic Factor/*chemistry
MH  - Cell Line, Tumor
MH  - Circular Dichroism
MH  - Gold/*chemistry
MH  - Humans
MH  - Molecular Dynamics Simulation
MH  - Molecular Structure
MH  - Nerve Growth Factor/*chemistry
MH  - Peptides/chemical synthesis/*chemistry
MH  - Quartz Crystal Microbalance Techniques
MH  - Surface Properties
EDAT- 2013/12/07 06:00
MHDA- 2014/09/30 06:00
CRDT- 2013/12/06 06:00
PHST- 2013/12/06 06:00 [entrez]
PHST- 2013/12/07 06:00 [pubmed]
PHST- 2014/09/30 06:00 [medline]
AID - 10.1039/c3cp52499j [doi]
PST - ppublish
SO  - Phys Chem Chem Phys. 2014 Jan 28;16(4):1536-44. doi: 10.1039/c3cp52499j.