PMID- 24309499
OWN - NLM
STAT- MEDLINE
DCOM- 20140708
LR  - 20211021
IS  - 2041-1723 (Electronic)
IS  - 2041-1723 (Linking)
VI  - 4
DP  - 2013
TI  - miRNomes of haematopoietic stem cells and dendritic cells identify miR-30b as a 
      regulator of Notch1.
PG  - 2903
LID - 10.1038/ncomms3903 [doi]
LID - 2903
AB  - Dendritic cells (DCs) are critical to initiate the immune response and maintain 
      tolerance, depending on different status and subsets. The expression profiles of 
      microRNAs (miRNAs) in various DC subsets and haematopoietic stem cells (HSCs), 
      which generate DCs, remain to be fully identified. Here we examine miRNomes of 
      mouse bone marrow HSCs, immature DCs, mature DCs and IL-10/NO-producing 
      regulatory DCs by deep sequencing. We identify numerous stage-specific miRNAs and 
      histone modification in HSCs and DCs at different differentiation stages. 
      miR-30b, significantly upregulated via a TGF-beta/Smad3-mediated epigenetic 
      pathway in regulatory DCs, can target Notch1 to promote IL-10 and NO production, 
      suggesting that miR-30b is a negative regulator of immune response. We also 
      identify miRNomes of in vivo counterparts of mature DCs and regulatory DCs and 
      systematically compare them with DCs cultured in vitro. These results provide a 
      resource for studying roles of miRNAs in stem cell biology, development and 
      functional regulation of DC subsets.
FAU - Su, Xiaoping
AU  - Su X
AD  - 1] National Key Laboratory of Medical Immunology and Institute of Immunology, 
      Second Military Medical University, Shanghai 200433, China [2].
FAU - Qian, Cheng
AU  - Qian C
FAU - Zhang, Qian
AU  - Zhang Q
FAU - Hou, Jin
AU  - Hou J
FAU - Gu, Yan
AU  - Gu Y
FAU - Han, Yanmei
AU  - Han Y
FAU - Chen, Yongjian
AU  - Chen Y
FAU - Jiang, Minghong
AU  - Jiang M
FAU - Cao, Xuetao
AU  - Cao X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nat Commun
JT  - Nature communications
JID - 101528555
RN  - 0 (Histones)
RN  - 0 (IL10 protein, mouse)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn30d microRNA, mouse)
RN  - 0 (Notch1 protein, mouse)
RN  - 0 (Receptor, Notch1)
RN  - 0 (Smad3 Protein)
RN  - 0 (Smad3 protein, mouse)
RN  - 0 (Transforming Growth Factor beta)
RN  - 130068-27-8 (Interleukin-10)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Dendritic Cells/cytology/*physiology
MH  - Epigenesis, Genetic
MH  - Gene Expression Regulation
MH  - Hematopoietic Stem Cells/cytology/*physiology
MH  - Histones/metabolism
MH  - Interleukin-10/genetics/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/*genetics/metabolism
MH  - Nitric Oxide/metabolism
MH  - Receptor, Notch1/genetics/*metabolism
MH  - Smad3 Protein/metabolism
MH  - Transforming Growth Factor beta/metabolism
PMC - PMC3863901
EDAT- 2013/12/07 06:00
MHDA- 2014/07/09 06:00
CRDT- 2013/12/07 06:00
PHST- 2013/05/17 00:00 [received]
PHST- 2013/11/07 00:00 [accepted]
PHST- 2013/12/07 06:00 [entrez]
PHST- 2013/12/07 06:00 [pubmed]
PHST- 2014/07/09 06:00 [medline]
AID - ncomms3903 [pii]
AID - 10.1038/ncomms3903 [doi]
PST - ppublish
SO  - Nat Commun. 2013;4:2903. doi: 10.1038/ncomms3903.