PMID- 24312787
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20131206
LR  - 20240318
IS  - 2228-5881 (Print)
IS  - 2251-7308 (Electronic)
IS  - 2228-5881 (Linking)
VI  - 2
IP  - 2
DP  - 2012
TI  - Development of a dispersive liquid-liquid microextraction technique for the 
      extraction and spectrofluorimetric determination of fluoxetine in pharmaceutical 
      formulations and human urine.
PG  - 157-64
LID - 10.5681/apb.2012.024 [doi]
AB  - PURPOSE: Fluoxetine is the most prescribed antidepressant drug worldwide. In this 
      work, a new dispersive liquid-liquid microextraction (DLLME) method combined with 
      spectrofluorimetry has been developed for the extraction and determination of FLX 
      in pharmaceutical formulations and human urine. METHODS: For FLX determination, 
      the pH of a 10 mL of sample solution containing FLX, was adjusted to 11.0. Then, 
      800 microL of ethanol containing 100 microL of chloroform was injected rapidly into the 
      sample solution. A cloudy solution was formed and FLX extracted into the fine 
      droplets of chloroform. After centrifugation, the extraction solvent was 
      sedimented and supernatant aqueous phase was readily decanted. The remained 
      organic phase was diluted with ethanol and its fluorescence was measured at 292+/-3 
      nm after excitation at 234+/-3 nm. RESULTS: Some important parameters influencing 
      microextraction efficiency were investigated. Under the optimum extraction 
      conditions, a linear calibration curve in the range of 10 to 800 ng/mL with a 
      correlation coefficient of r(2) = 0.9993 was obtained. Limit of detection (LOD) 
      and limit of quantification (LOQ) were found to be 2.78 and 9.28 ng/mL, 
      respectively. The relative standard deviations (RSDs) were less than 4%. Average 
      recoveries for spiked samples were 93-104%. CONCLUSION: The proposed method gives 
      a very rapid, simple, sensitive, wide dynamic range and low-cost procedure for 
      the determination of FLX.
FAU - Bavili Tabrizi, Ahad
AU  - Bavili Tabrizi A
AD  - Department of Medicinal Chemistry, Faculty of Pharmacy and Biotechnology Research 
      Center, Tabriz University of Medical Sciences, Tabriz, Iran.
FAU - Rezazadeh, Ahmad
AU  - Rezazadeh A
LA  - eng
PT  - Journal Article
DEP - 20120615
PL  - Iran
TA  - Adv Pharm Bull
JT  - Advanced pharmaceutical bulletin
JID - 101578021
PMC - PMC3845995
OTO - NOTNLM
OT  - Dispersive liquid-liquid microextraction
OT  - Fluoxetine
OT  - Injectable formulation
OT  - Spectrofluorimetry
OT  - Urine
EDAT- 2012/01/01 00:00
MHDA- 2012/01/01 00:01
PMCR- 2012/12/01
CRDT- 2013/12/07 06:00
PHST- 2012/05/20 00:00 [received]
PHST- 2012/06/10 00:00 [accepted]
PHST- 2013/12/07 06:00 [entrez]
PHST- 2012/01/01 00:00 [pubmed]
PHST- 2012/01/01 00:01 [medline]
PHST- 2012/12/01 00:00 [pmc-release]
AID - 10.5681/apb.2012.024 [doi]
PST - ppublish
SO  - Adv Pharm Bull. 2012;2(2):157-64. doi: 10.5681/apb.2012.024. Epub 2012 Jun 15.