PMID- 24313705
OWN - NLM
STAT- MEDLINE
DCOM- 20140807
LR  - 20211021
IS  - 1535-4989 (Electronic)
IS  - 1044-1549 (Print)
IS  - 1044-1549 (Linking)
VI  - 50
IP  - 5
DP  - 2014 May
TI  - Airway contractility in the precision-cut lung slice after cryopreservation.
PG  - 876-81
LID - 10.1165/rcmb.2013-0166MA [doi]
AB  - An emerging tool in airway biology is the precision-cut lung slice (PCLS). 
      Adoption of the PCLS as a model for assessing airway reactivity has been hampered 
      by the limited time window within which tissues remain viable. Here we 
      demonstrate that the PCLS can be frozen, stored long-term, and then thawed for 
      later experimental use. Compared with the never-frozen murine PCLS, the 
      frozen-thawed PCLS shows metabolic activity that is decreased to an extent 
      comparable to that observed in other cryopreserved tissues but shows no 
      differences in cell viability or in airway caliber responses to the contractile 
      agonist methacholine or the relaxing agonist chloroquine. These results indicate 
      that freezing and long-term storage is a feasible solution to the problem of 
      limited viability of the PCLS in culture.
FAU - Rosner, Sonia R
AU  - Rosner SR
AD  - 1 Department of Environmental Health, Harvard School of Public Health, Boston, 
      Massachusetts.
FAU - Ram-Mohan, Sumati
AU  - Ram-Mohan S
FAU - Paez-Cortez, Jesus R
AU  - Paez-Cortez JR
FAU - Lavoie, Tera L
AU  - Lavoie TL
FAU - Dowell, Maria L
AU  - Dowell ML
FAU - Yuan, Lei
AU  - Yuan L
FAU - Ai, Xingbin
AU  - Ai X
FAU - Fine, Alan
AU  - Fine A
FAU - Aird, William C
AU  - Aird WC
FAU - Solway, Julian
AU  - Solway J
FAU - Fredberg, Jeffrey J
AU  - Fredberg JJ
FAU - Krishnan, Ramaswamy
AU  - Krishnan R
LA  - eng
GR  - P01 HL120839/HL/NHLBI NIH HHS/United States
GR  - R01 HL116163/HL/NHLBI NIH HHS/United States
GR  - R21 HL112619/HL/NHLBI NIH HHS/United States
GR  - 1R21HL112619/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Respir Cell Mol Biol
JT  - American journal of respiratory cell and molecular biology
JID - 8917225
SB  - IM
MH  - Animals
MH  - Cell Death/physiology
MH  - Cell Survival/physiology
MH  - Cryopreservation/methods
MH  - Freezing
MH  - Lung/*physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Muscle Contraction/*physiology
PMC - PMC4068941
EDAT- 2013/12/10 06:00
MHDA- 2014/08/08 06:00
PMCR- 2015/05/01
CRDT- 2013/12/10 06:00
PHST- 2013/12/10 06:00 [entrez]
PHST- 2013/12/10 06:00 [pubmed]
PHST- 2014/08/08 06:00 [medline]
PHST- 2015/05/01 00:00 [pmc-release]
AID - 10.1165/rcmb.2013-0166MA [doi]
PST - ppublish
SO  - Am J Respir Cell Mol Biol. 2014 May;50(5):876-81. doi: 10.1165/rcmb.2013-0166MA.