PMID- 24314935
OWN - NLM
STAT- MEDLINE
DCOM- 20140731
LR  - 20220331
IS  - 1873-2623 (Electronic)
IS  - 0041-1345 (Linking)
VI  - 45
IP  - 10
DP  - 2013
TI  - Toll-like receptor 4 and CD14 gene polymorphisms in Tunisian kidney 
      transplantation.
PG  - 3472-7
LID - S0041-1345(13)00913-5 [pii]
LID - 10.1016/j.transproceed.2013.09.003 [doi]
AB  - BACKGROUND: Acute and chronic rejections remain an important cause of graft loss 
      after renal transplantation. Currently, activation of innate immune responses 
      through Toll-like receptors (TLRs) is suspected to be implied in the loss of the 
      transplant tolerance. OBJECTIVES: We investigated functional single nucleotide 
      polymorphisms (SNPs) of TLR4 and its coreceptor CD14 in kidney transplantation 
      and looked for any potential role in acute rejection (AR) and chronic allograft 
      nephropathy (CAN) and impact on graft survival. PATIENTS AND METHODS: TLR4 
      (Asp299Gly) and CD14 (C/T -159) SNPs were detected using polymerase chain 
      reaction-restriction fragment length polymorphism (PCR-RFLP) in 209 kidney 
      transplant recipients (KTRs) including 132 treated with mycophenolate mofetil 
      (MMF+). AR occurred in 59 patients and 24 were identified as having CAN by biopsy 
      and scored according to the Banff criteria. RESULTS: There were no significant 
      associations between TLR4 and CD14 genotypes and alleles and the occurrence of 
      both AR episodes and CAN. Moreover, TLR4 and CD14 SNPs did not seem to influence 
      kidney graft survival. Analysis according to human leukocyte antigen (HLA) 
      compatibility status, positivity of anti-HLA antibodies, and immunosuppression by 
      MMF confirmed the absence of correlation of the investigated SNPs with the graft 
      outcome. In addition, incidence of post-transplantation infections, including 
      cytomegalovirus (CMV) infections, was not influenced by both TLR4 and CD14 SNPs. 
      CONCLUSION: These results suggest that TLR4 (Asp299Gly) and CD14 (C/T -159) 
      functional SNPs do not play a major role in AR, CAN, and kidney graft survival. 
      Therefore, intragraft monitoring of TLR4/CD14 genes expression by messenger RNA 
      (mRNA) would provide clarity on the exact role of these receptors in graft 
      injuries.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Krichen, H
AU  - Krichen H
AD  - Laboratory of Research in Immunology of Renal Transplantation and Immunopathology 
      (LR03SP01), University Tunis El Manar, Charles Nicolle Hospital, Tunis, Tunisia.
FAU - Gorgi, Y
AU  - Gorgi Y
FAU - Dhaouadi, T
AU  - Dhaouadi T
FAU - Mecheri, Y
AU  - Mecheri Y
FAU - Sfar, I
AU  - Sfar I
FAU - Bardi, R
AU  - Bardi R
FAU - Bacha, M M
AU  - Bacha MM
FAU - Abderrahim, E
AU  - Abderrahim E
FAU - Jendoubi-Ayed, S
AU  - Jendoubi-Ayed S
FAU - Ayed, K
AU  - Ayed K
FAU - Ben Abdallah, T
AU  - Ben Abdallah T
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
RN  - 0 (Antibodies)
RN  - 0 (HLA Antigens)
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
SB  - IM
MH  - Acute Disease
MH  - Adult
MH  - Antibodies/blood
MH  - Chronic Disease
MH  - Communicable Diseases/genetics/immunology
MH  - Female
MH  - Gene Frequency
MH  - Genetic Predisposition to Disease
MH  - Graft Rejection/*genetics/immunology
MH  - Graft Survival/genetics
MH  - HLA Antigens/immunology
MH  - Humans
MH  - Immunity, Innate/genetics
MH  - Immunosuppressive Agents/therapeutic use
MH  - Kidney Transplantation/*adverse effects
MH  - Lipopolysaccharide Receptors/*genetics
MH  - Male
MH  - Middle Aged
MH  - Phenotype
MH  - *Polymorphism, Single Nucleotide
MH  - Risk Factors
MH  - Toll-Like Receptor 4/*genetics
MH  - Treatment Outcome
MH  - Tunisia
MH  - Young Adult
EDAT- 2013/12/10 06:00
MHDA- 2014/08/01 06:00
CRDT- 2013/12/10 06:00
PHST- 2013/12/10 06:00 [entrez]
PHST- 2013/12/10 06:00 [pubmed]
PHST- 2014/08/01 06:00 [medline]
AID - S0041-1345(13)00913-5 [pii]
AID - 10.1016/j.transproceed.2013.09.003 [doi]
PST - ppublish
SO  - Transplant Proc. 2013;45(10):3472-7. doi: 10.1016/j.transproceed.2013.09.003.