PMID- 24315849 OWN - NLM STAT- MEDLINE DCOM- 20140917 LR - 20161125 IS - 1872-7573 (Electronic) IS - 0378-8741 (Linking) VI - 151 IP - 2 DP - 2014 Feb 3 TI - Proteomic profiling and post-translational modifications in human keratinocytes treated with Mucuna pruriens leaf extract. PG - 873-81 LID - S0378-8741(13)00857-X [pii] LID - 10.1016/j.jep.2013.11.053 [doi] AB - ETHNOPHARMACOLOGICAL RELEVANCE: Mucuna pruriens (Mp) is a plant belonging to the Fabaceae family, with several medicinal properties among which its potential to treat diseases where reactive oxygen species (ROS) play an important role in the pathogeneses. The aim was to investigate the effects of Mp leaf methanolic extract (MPME) on human keratinocytes protein expression and its role in preventing proteins oxidation after oxidative stress (OS) exposure. MATERIAL AND METHODS: The effects of MPME on HaCaT cells protein expression were evaluated treating cells with different concentrations of MPME, with glucose oxidase (GO, source of OS) and with MPME subsequently treated with GO. The protein patterns of treated HaCaT cells are analyzed by two-dimensional gel electrophoresis (2-DE) and compared with that of untreated HaCaT. Immunoblotting was then used to evaluate the role of MPME in preventing the 4-hydroxynonenal protein adducts (4-HNE PAs) formation (marker of OS). RESULTS: Eighteen proteins, identified by mass spectrometry (LC-ESI-CID-MS/MS), were modulated distinctly by MPME in HaCaT. Overall, MPME counteract GO effect, reducing the GO-induced overexpression of several proteins involved in stress response (T-complex protein 1, Protein disulfide-isomerase A3, Protein DJ-1, and Stress-induced-phosphoprotein 1), in cell energy methabolism (Inorganic pyrophosphatase, Triosephosphate isomerase isoform 1, 2-phosphopyruvate-hydratase alpha-enolase, and Fructose-bisphosphate aldolase A isoform 1), in cytoskeletal organization (Cytokeratins 18, 9, 2, Cofilin-1, Annexin A2 and F-actin-capping protein subunit beta isoform 1) and in cell cycle progression (Eukaryotic translation initiation factor 5A-1 isoform B). In addition, MPME decreased the 4-HNE PAs levels, in particular on 2-phosphopyruvate-hydratase alpha-enolase and Cytokeratin 9. CONCLUSIONS: Our findings show that MPME might be helpful in the treatment of OS-related skin diseases by preventing protein post-translational modifications (4-HNE PAs). CI - (c) 2013 Published by Elsevier Ireland Ltd. FAU - Cortelazzo, Alessio AU - Cortelazzo A AD - Department of Medical Biotechnologies, University of Siena, Siena, Italy; Child Neuropsychiatry Unit, University Hospital AOUS, Siena, Italy. FAU - Lampariello, Raffaella L AU - Lampariello RL AD - Department of Biotechnology, Chemistry and Pharmaceutical Sciences, University of Siena, Siena, Italy. FAU - Sticozzi, Claudia AU - Sticozzi C AD - Department of Life Sciences and Biotechnologies, University of Ferrara, Via Luigi Borsari 46, 44100 Ferrara, Italy. FAU - Guerranti, Roberto AU - Guerranti R AD - Department of Medical Biotechnologies, University of Siena, Siena, Italy. FAU - Mirasole, Cristiana AU - Mirasole C AD - Department of Ecological and Biological Sciences, University of Tuscia, Viterbo, Italy. FAU - Zolla, Lello AU - Zolla L AD - Department of Ecological and Biological Sciences, University of Tuscia, Viterbo, Italy. FAU - Sacchetti, Gianni AU - Sacchetti G AD - Department of Life Sciences and Biotechnologies, University of Ferrara, Via Luigi Borsari 46, 44100 Ferrara, Italy. FAU - Hajek, Joussef AU - Hajek J AD - Child Neuropsychiatry Unit, University Hospital AOUS, Siena, Italy. FAU - Valacchi, Giuseppe AU - Valacchi G AD - Department of Life Sciences and Biotechnologies, University of Ferrara, Via Luigi Borsari 46, 44100 Ferrara, Italy; Department of Food and Nutrition, Kyung Hee University, Seoul, Rrepbulic of Korea. Electronic address: giuseppe.valacchi@unife.it. LA - eng PT - Journal Article DEP - 20131205 PL - Ireland TA - J Ethnopharmacol JT - Journal of ethnopharmacology JID - 7903310 RN - 0 (Aldehydes) RN - 0 (Plant Extracts) RN - 0 (Proteins) RN - EC 1.1.3.4 (Glucose Oxidase) RN - K1CVM13F96 (4-hydroxy-2-nonenal) SB - IM MH - Aldehydes/metabolism MH - Cell Line MH - Glucose Oxidase/pharmacology MH - Humans MH - Keratinocytes/*drug effects/metabolism MH - *Mucuna MH - Oxidative Stress MH - Plant Extracts/*pharmacology MH - Plant Leaves MH - Protein Binding MH - Protein Processing, Post-Translational/*drug effects MH - Proteins/metabolism MH - Proteomics OTO - NOTNLM OT - Keratinocytes OT - Leaf extract OT - Mucuna pruriens OT - Oxidative stress OT - Proteomics EDAT- 2013/12/10 06:00 MHDA- 2014/09/18 06:00 CRDT- 2013/12/10 06:00 PHST- 2013/09/18 00:00 [received] PHST- 2013/11/05 00:00 [revised] PHST- 2013/11/27 00:00 [accepted] PHST- 2013/12/10 06:00 [entrez] PHST- 2013/12/10 06:00 [pubmed] PHST- 2014/09/18 06:00 [medline] AID - S0378-8741(13)00857-X [pii] AID - 10.1016/j.jep.2013.11.053 [doi] PST - ppublish SO - J Ethnopharmacol. 2014 Feb 3;151(2):873-81. doi: 10.1016/j.jep.2013.11.053. Epub 2013 Dec 5.