PMID- 24316452
OWN - NLM
STAT- MEDLINE
DCOM- 20140916
LR  - 20191210
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 54
DP  - 2014 Apr 15
TI  - A simple and portable device for the quantification of TNF-alpha in human plasma by 
      means of on-chip magnetic bead-based proximity ligation assay.
PG  - 499-505
LID - S0956-5663(13)00741-0 [pii]
LID - 10.1016/j.bios.2013.10.039 [doi]
AB  - There is a general need in healthcare systems all around the world to reduce 
      costs in terms of time and money without compromising patients outcome. 
      Point-of-Care Testing (POCT) is currently being used in some applications (e.g. 
      POC coagulation devices) as an alternative to already established standard 
      central laboratory tests to overcome sample transportation and long turnaround 
      times. The main objective of this investigation was to quantify Tumour Necrosis 
      Factor-alpha (TNF-alpha) on-chip within the clinical relevant range of 5-100 pg/mL in 
      human pooled plasma. The novel solid-phase assay developed in this study was a 
      magnetic bead-based proximity ligation assay (PLA) in which one of the assay 
      proximity probes was directly immobilised onto streptavidin-coated magnetic 
      beads. The portable device was based on a disposable and single-use cyclo-olefin 
      polymer (COP) microfluidic chip interfaced with a quantitative real-time 
      polymerase chain reaction (qPCR) device previously developed in-house. Sample 
      volume was 10 microL and total assay time under 3 h. The POC device and assay 
      developed offer portability, smaller reagent and sample consumption, and faster 
      time-to-results compared with standard ELISAs. Determination and monitoring of 
      TNF-alpha therapy at the point-of-care will help to improve clinical and/or 
      economical outcome in governmental healthcare budgets.
CI  - (c) 2013 Elsevier B.V. All rights reserved.
FAU - Castro-Lopez, Vanessa
AU  - Castro-Lopez V
AD  - CIC microGUNE, Goiru kalea 9, 20500 Arrasate-Mondragon, Gipuzkoa, Spain. 
      Electronic address: vcastro@cicmicrogune.es.
FAU - Elizalde, Jorge
AU  - Elizalde J
AD  - CIC microGUNE, Goiru kalea 9, 20500 Arrasate-Mondragon, Gipuzkoa, Spain; 
      IK4-Ikerlan, P(o) JM feminine Arizmendiarrieta 2, 20500 Arrasate-Mondragon, Gipuzkoa, 
      Spain.
FAU - Pacek, Marcin
AU  - Pacek M
AD  - POC Microsolutions, Goiru kalea 9, 20500 Arrasate-Mondragon, Gipuzkoa, Spain.
FAU - Hijona, Elizabeth
AU  - Hijona E
AD  - Department of Gastroenterology, Hospital Donostia/Instituto Biodonostia, Centro 
      de Investigacion Biomedica en Red de Enfermedades Hepaticas y Digestivas 
      (CIBERehd), Universidad del Pais Vasco UPV/EHU, San Sebastian, Spain.
FAU - Bujanda, Luis
AU  - Bujanda L
AD  - Department of Gastroenterology, Hospital Donostia/Instituto Biodonostia, Centro 
      de Investigacion Biomedica en Red de Enfermedades Hepaticas y Digestivas 
      (CIBERehd), Universidad del Pais Vasco UPV/EHU, San Sebastian, Spain.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131106
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Biosensing Techniques/instrumentation
MH  - Equipment Design
MH  - Humans
MH  - Limit of Detection
MH  - Microfluidic Analytical Techniques/*instrumentation
MH  - *Point-of-Care Systems
MH  - Real-Time Polymerase Chain Reaction/instrumentation
MH  - Tumor Necrosis Factor-alpha/*blood
OTO - NOTNLM
OT  - COP
OT  - Microfluidic chip
OT  - Point-of-care
OT  - Solid-phase proximity ligation assay
OT  - TNF-alpha
OT  - qPCR
EDAT- 2013/12/10 06:00
MHDA- 2014/09/17 06:00
CRDT- 2013/12/10 06:00
PHST- 2013/07/29 00:00 [received]
PHST- 2013/10/11 00:00 [revised]
PHST- 2013/10/22 00:00 [accepted]
PHST- 2013/12/10 06:00 [entrez]
PHST- 2013/12/10 06:00 [pubmed]
PHST- 2014/09/17 06:00 [medline]
AID - S0956-5663(13)00741-0 [pii]
AID - 10.1016/j.bios.2013.10.039 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2014 Apr 15;54:499-505. doi: 10.1016/j.bios.2013.10.039. 
      Epub 2013 Nov 6.