PMID- 24316858
OWN - NLM
STAT- MEDLINE
DCOM- 20140930
LR  - 20220408
IS  - 1678-2674 (Electronic)
IS  - 0102-8650 (Linking)
VI  - 28
IP  - 12
DP  - 2013 Dec
TI  - Expression of oxidative stress and antioxidant defense genes in the kidney of 
      inbred mice after intestinal ischemia and reperfusion.
PG  - 848-55
LID - S0102-86502013001200007 [pii]
AB  - PURPOSE: To determine the gene expressions profile related to the oxidative 
      stress and the antioxidant response in the kidneys of mice subjected to 
      intestinal ischemia and reperfusion. METHODS: Twelve inbred mice (C57BL/6) were 
      randomly assigned to one of two groups: the control group (CG) underwent 
      anesthesia and was observed for 120 min and the ischemia/reperfusion group (IRG), 
      animals were anesthetized and subjected to laparotomy and ischemia for 60 minutes 
      followed by 60 minutes of reperfusion. The expressions of 84 genes from the 
      kidney were determined by the Reverse Transcription qualitative Polymerase Chain 
      Reaction (RT-qPCR). All genes that were up regulated by more than threefold using 
      the algorithm [2(DeltaDeltaCt)] were considered statically significant (p<0.05). RESULTS: 
      In the IRG group 29 (34.52%) of 84 genes, were up regulated by more than 
      threefold. The genes that were differentially up regulated in the glutathione 
      peroxidase cluster (10 genes): were Gpx2 and Gpx7. The genes that were up 
      regulated in the peroxidase cluster (16 genes) were following: Duox1, Epx, Lpo, 
      Mpo, Ptgs2, Rag2, Serpinb1b, Tmod1 and Tpo. The genes that up regulated in the 
      reactive oxygen species cluster (16 genes): Il19, Il22, Nos2, Nox1, Noxa1, Noxo1, 
      Recql4 and Sod2. The genes that were up regulated in the oxidative stress cluster 
      (22 genes) were: Mpp4, Nudt15, Upc3 and Xpa. The genes that were up regulated in 
      the oxygen carriers cluster (12 genes) were: Hbq1, Mb, Ngb, Slc38a1 and Xirp1. 
      The peroxiredoxins genes (10) showed no consistent differential regulation. 
      CONCLUSION: The genes related to oxidative stress and antioxidant defense showed 
      increased expression in renal tissue trigged intestinal ischemia and reperfusion.
FAU - Teruya, Roberto
AU  - Teruya R
FAU - Ikejiri, Adauto Tsutomu
AU  - Ikejiri AT
FAU - Somaio Neto, Frederico
AU  - Somaio Neto F
FAU - Chaves, Jose Carlos
AU  - Chaves JC
FAU - Bertoletto, Paulo Roberto
AU  - Bertoletto PR
FAU - Taha, Murched Omar
AU  - Taha MO
FAU - Fagundes, Djalma Jose
AU  - Fagundes DJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Brazil
TA  - Acta Cir Bras
JT  - Acta cirurgica brasileira
JID - 9103983
RN  - 0 (Antioxidants)
SB  - IM
MH  - Animals
MH  - Antioxidants/metabolism
MH  - Down-Regulation/genetics
MH  - Gene Expression/*genetics
MH  - *Intestine, Small/metabolism/physiopathology
MH  - *Kidney/metabolism/physiopathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Oxidative Stress/*genetics
MH  - Random Allocation
MH  - Reperfusion Injury/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Time Factors
MH  - Up-Regulation/genetics
EDAT- 2013/12/10 06:00
MHDA- 2014/10/01 06:00
CRDT- 2013/12/10 06:00
PHST- 2013/08/26 00:00 [received]
PHST- 2013/11/26 00:00 [accepted]
PHST- 2013/12/10 06:00 [entrez]
PHST- 2013/12/10 06:00 [pubmed]
PHST- 2014/10/01 06:00 [medline]
AID - S0102-86502013001200007 [pii]
AID - 10.1590/s0102-86502013001200007 [doi]
PST - ppublish
SO  - Acta Cir Bras. 2013 Dec;28(12):848-55. doi: 10.1590/s0102-86502013001200007.