PMID- 24321744
OWN - NLM
STAT- MEDLINE
DCOM- 20140306
LR  - 20161125
IS  - 1879-3169 (Electronic)
IS  - 0378-4274 (Linking)
VI  - 225
IP  - 1
DP  - 2014 Feb 10
TI  - Prenatal buprenorphine exposure decreases neurogenesis in rats.
PG  - 92-101
LID - S0378-4274(13)01457-4 [pii]
LID - 10.1016/j.toxlet.2013.12.001 [doi]
AB  - Perinatal opioid exposure has a negative effect on neurogenesis and produces 
      neurological consequences. However, its mechanisms of action are incompletely 
      understood. Buprenorphine, a mixed opioid agonist/antagonist, is an alternative 
      medication for managing pregnant opioid addicts. This study provides evidence of 
      decreased neurogenesis and depression-like consequences following prenatal 
      exposure to buprenorphine and sheds light on mechanisms of action in a rat model 
      involving administration of intraperitoneal injection to pregnant rats starting 
      from gestation day 7 and lasting for 14 days and a cultured neurosphere model. 
      Results of forced swimming test and tail suspension test showed that pups at 
      postnatal day 21 had worse parameters of depression-like neurobehaviors, 
      independent of gender. Neurobehavioral changes were accompanied by reduction of 
      neuronal composition, biochemical parameters of neural stem/progenitor cells, 
      brain-derived neurotrophic factor (BDNF) expression, tropomyosin-related kinase 
      receptor type B phosphorylation, protein kinase A (PKA) activity, and cAMP 
      response element-binding protein phosphorylation. Results of parallel cell 
      studies further demonstrated a negative impact of buprenorphine on cultured 
      neurospheres, including proliferation, differentiation, BDNF expression and 
      signaling, and PKA activity. Taken together, our results suggest that prenatal 
      exposure to buprenorphine might result in depression-like phenotypes associated 
      with impaired BDNF action and decreased neurogenesis in the developing brain of 
      weanlings.
CI  - Copyright (c) 2013 Elsevier Ireland Ltd. All rights reserved.
FAU - Wu, Chih-Cheng
AU  - Wu CC
AD  - Department of Anesthesiology, Taichung Veterans General Hospital, Taichung 407, 
      Taiwan; Department of Financial and Computational Mathematics, Providence 
      University, Taichung 433, Taiwan.
FAU - Hung, Chih-Jen
AU  - Hung CJ
AD  - Department of Anesthesiology, Taichung Veterans General Hospital, Taichung 407, 
      Taiwan; Graduate School of Nursing, HungKuang University, Taichung 433, Taiwan.
FAU - Shen, Ching-Hui
AU  - Shen CH
AD  - Department of Anesthesiology, Taichung Veterans General Hospital, Taichung 407, 
      Taiwan.
FAU - Chen, Wen-Ying
AU  - Chen WY
AD  - Department of Veterinary Medicine, National Chung Hsing University, Taichung 402, 
      Taiwan.
FAU - Chang, Cheng-Yi
AU  - Chang CY
AD  - Department of Surgery, Feng Yuan Hospital, Taichung 420, Taiwan.
FAU - Pan, Hung-Chuan
AU  - Pan HC
AD  - Department of Neurosurgery, Taichung Veterans General Hospital, Taichung 407, 
      Taiwan; Faculty of Medicine, School of Medicine, National Yang-Ming University, 
      Taipei 112, Taiwan.
FAU - Liao, Su-Lan
AU  - Liao SL
AD  - Department of Education and Research, Taichung Veterans General Hospital, 
      Taichung 407, Taiwan.
FAU - Chen, Chun-Jung
AU  - Chen CJ
AD  - Graduate School of Nursing, HungKuang University, Taichung 433, Taiwan; 
      Department of Education and Research, Taichung Veterans General Hospital, 
      Taichung 407, Taiwan; Institute of Biomedical Sciences, National Chung Hsing 
      University, Taichung 402, Taiwan; Center for General Education, Tunghai 
      University, Taichung 407, Taiwan. Electronic address: cjchen@vghtc.gov.tw.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131207
PL  - Netherlands
TA  - Toxicol Lett
JT  - Toxicology letters
JID - 7709027
RN  - 0 (Analgesics, Opioid)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 40D3SCR4GZ (Buprenorphine)
RN  - EC 2.3.1.48 (CREB-Binding Protein)
RN  - EC 2.3.1.48 (Crebbp protein, rat)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
SB  - IM
MH  - Analgesics, Opioid/administration & dosage/*toxicity
MH  - Animals
MH  - Behavior, Animal/drug effects
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Buprenorphine/administration & dosage/*toxicity
MH  - CREB-Binding Protein/metabolism
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Cyclic AMP-Dependent Protein Kinases/metabolism
MH  - Depression/chemically induced/physiopathology/psychology
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Gestational Age
MH  - Injections, Intraperitoneal
MH  - Male
MH  - Maternal Exposure
MH  - Motor Activity/drug effects
MH  - Nervous System/*drug effects/growth & 
      development/metabolism/pathology/physiopathology
MH  - Neurogenesis/*drug effects
MH  - Neurons/*drug effects/metabolism/pathology
MH  - Phosphorylation
MH  - Pregnancy
MH  - *Prenatal Exposure Delayed Effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, trkB/drug effects/metabolism
MH  - Signal Transduction/drug effects
MH  - Spheroids, Cellular
MH  - Swimming
MH  - Time Factors
OTO - NOTNLM
OT  - Depression
OT  - Neurogenesis
OT  - Neurotrophin
OT  - Opioid
EDAT- 2013/12/11 06:00
MHDA- 2014/03/07 06:00
CRDT- 2013/12/11 06:00
PHST- 2013/09/12 00:00 [received]
PHST- 2013/11/05 00:00 [revised]
PHST- 2013/12/02 00:00 [accepted]
PHST- 2013/12/11 06:00 [entrez]
PHST- 2013/12/11 06:00 [pubmed]
PHST- 2014/03/07 06:00 [medline]
AID - S0378-4274(13)01457-4 [pii]
AID - 10.1016/j.toxlet.2013.12.001 [doi]
PST - ppublish
SO  - Toxicol Lett. 2014 Feb 10;225(1):92-101. doi: 10.1016/j.toxlet.2013.12.001. Epub 
      2013 Dec 7.