PMID- 24323361
OWN - NLM
STAT- MEDLINE
DCOM- 20140925
LR  - 20211021
IS  - 1559-1166 (Electronic)
IS  - 0895-8696 (Linking)
VI  - 52
IP  - 1
DP  - 2014 Jan
TI  - Influence of terminal differentiation and PACAP on the cytokine, chemokine, and 
      growth factor secretion of mammary epithelial cells.
PG  - 28-36
LID - 10.1007/s12031-013-0193-3 [doi]
AB  - Pituitary adenylate cyclase-activating polypeptide (PACAP), a neuropeptide with 
      trophic and cytoprotective effects, has been shown to affect cell survival, 
      proliferation, and also differentiation of various cell types. The high PACAP 
      level in the milk and its changes during lactation suggest a possible effect of 
      PACAP on the differentiation of mammary epithelial cells. Mammary cell 
      differentiation is regulated by hormones, growth factors, cytokines/chemokines, 
      and angiogenic proteins. In this study, differentiation was hormonally induced by 
      lactogenic hormones in confluent cultures of HC11 mouse mammary epithelial cells. 
      We investigated the effect of PACAP on mammary cell differentiation as well as 
      release of cytokines, chemokines, and growth factors. Differentiation was 
      assessed by expression analysis of the milk protein beta-casein. Differentiation 
      significantly decreased the secretion of interferon gammainduced protein (IP)-10, 
      "regulated upon activation normal T cell expressed and presumably secreted" 
      (RANTES), insulin-like growth factor-binding protein (IGFBP)-3 and the epidermal 
      growth factor receptor (EGFR) ligands, such as epidermal growth factor (EGF) and 
      amphiregulin (AREG). The changes in the levels of IP-10 and RANTES may be 
      relevant for the alterations in homing of T cells and B cells at different stages 
      of mammary gland development, while the changes of the EGFR ligands may 
      facilitate the switch from proliferative to lactating stage. PACAP did not 
      modulate the expression of beta-casein or the activity of hormone-induced pathways 
      as determined by the analysis of phosphorylation of Akt, STAT5, and p38 MAPK. 
      However, PACAP decreased the release of EGF and AREG from non-differentiated 
      cells. This may influence the extracellular signal-related transactivation of 
      EGFR in the non-differentiated mammary epithelium and is considered to have an 
      impact on the modulation of oncogenic EGFR signaling in breast cancer.
FAU - Csanaky, Katalin
AU  - Csanaky K
AD  - Department of Anatomy, PTE-MTA "Lendulet" PACAP Research Team, University of 
      Pecs, Szigeti ut 12, Pecs, 7624, Hungary.
FAU - Doppler, Wolfgang
AU  - Doppler W
FAU - Tamas, Andrea
AU  - Tamas A
FAU - Kovacs, Krisztina
AU  - Kovacs K
FAU - Toth, Gabor
AU  - Toth G
FAU - Reglodi, Dora
AU  - Reglodi D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131210
PL  - United States
TA  - J Mol Neurosci
JT  - Journal of molecular neuroscience : MN
JID - 9002991
RN  - 0 (Amphiregulin)
RN  - 0 (Areg protein, mouse)
RN  - 0 (Caseins)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokine CXCL10)
RN  - 0 (Cxcl10 protein, mouse)
RN  - 0 (EGF Family of Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Growth Substances)
RN  - 0 (Insulin-Like Growth Factor Binding Protein 3)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Pituitary Adenylate Cyclase-Activating Polypeptide)
RN  - 0 (STAT5 Transcription Factor)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Amphiregulin
MH  - Animals
MH  - Caseins/genetics/metabolism
MH  - *Cell Differentiation
MH  - Cell Line
MH  - Chemokine CCL5/genetics/*metabolism
MH  - Chemokine CXCL10/genetics/*metabolism
MH  - EGF Family of Proteins
MH  - Epidermal Growth Factor/genetics/*metabolism
MH  - Epithelial Cells/cytology/drug effects/*metabolism
MH  - Glycoproteins/genetics/metabolism
MH  - Growth Substances/*pharmacology
MH  - Insulin-Like Growth Factor Binding Protein 3/genetics/metabolism
MH  - Intercellular Signaling Peptides and Proteins/genetics/metabolism
MH  - Mammary Glands, Animal/cytology
MH  - Mice
MH  - Pituitary Adenylate Cyclase-Activating Polypeptide/*pharmacology
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - STAT5 Transcription Factor/metabolism
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
EDAT- 2013/12/11 06:00
MHDA- 2014/09/26 06:00
CRDT- 2013/12/11 06:00
PHST- 2013/10/17 00:00 [received]
PHST- 2013/11/21 00:00 [accepted]
PHST- 2013/12/11 06:00 [entrez]
PHST- 2013/12/11 06:00 [pubmed]
PHST- 2014/09/26 06:00 [medline]
AID - 10.1007/s12031-013-0193-3 [doi]
PST - ppublish
SO  - J Mol Neurosci. 2014 Jan;52(1):28-36. doi: 10.1007/s12031-013-0193-3. Epub 2013 
      Dec 10.