PMID- 24323403
OWN - NLM
STAT- MEDLINE
DCOM- 20141030
LR  - 20220310
IS  - 1095-8355 (Electronic)
IS  - 1065-6995 (Print)
IS  - 1065-6995 (Linking)
VI  - 38
IP  - 4
DP  - 2014 Apr
TI  - Salidroside induces rat mesenchymal stem cells to differentiate into dopaminergic 
      neurons.
PG  - 462-71
LID - 10.1002/cbin.10217 [doi]
AB  - Parkinson's disease (PD) is a neurodegenerative disorder characterised by the 
      loss of substantia nigra dopaminergic neurons that leads to a reduction in 
      striatal dopamine (DA) levels. Replacing lost cells by transplanting dopaminergic 
      neurons has potential value to repair the damaged brain. Salidroside (SD), a 
      phenylpropanoid glycoside isolated from plant Rhodiola rosea, is neuroprotective. 
      We examined whether salidroside can induce mesenchymal stem cells (MSCs) to 
      differentiate into neuron-like cells, and convert MSCs into dopamine neurons that 
      can be applied in clinical use. Salidroside induced rMSCs to adopt a neuronal 
      morphology, upregulated the expression of neuronal marker molecules, such as 
      gamma neuronal enolase 2 (Eno2/NSE), microtubule-associated protein 2 (Map2), and 
      beta 3 class III tubulin (Tubb3/beta-tubulin III). It also increased expression of 
      brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and nerve growth 
      factor (NGF) mRNAs, and promoted the secretion of these growth factors. The 
      expression of dopamine neurons markers, such as dopamine-beta-hydroxy (DBH), dopa 
      decarboxylase (DDC) and tyrosine hydroxylase (TH), was significantly upregulated 
      after treatment with salidroside for 1-12 days. DA steadily increased after 
      treatment with salidroside for 1-6 days. Thus salidroside can induce rMSCs to 
      differentiate into dopaminergic neurons.
CI  - (c) 2014 The Authors Cell Biology International Published by John Wiley & Sons Ltd 
      on behalf of International Federation of Cell Biology.
FAU - Zhao, Hong-Bin
AU  - Zhao HB
AD  - Institute of Orthopedics, General Hospital of Lanzhou Military Command of the 
      PLA, Lanzhou, Gansu, 730050, China.
FAU - Ma, Hui
AU  - Ma H
FAU - Ha, Xiao-Qin
AU  - Ha XQ
FAU - Zheng, Ping
AU  - Zheng P
FAU - Li, Xiao-Yun
AU  - Li XY
FAU - Zhang, Ming
AU  - Zhang M
FAU - Dong, Ju-Zi
AU  - Dong JZ
FAU - Yang, Yin-Shu
AU  - Yang YS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140131
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Glucosides)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Mtap2 protein, mouse)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
RN  - 0 (Phenols)
RN  - 0 (RNA, Messenger)
RN  - M983H6N1S9 (rhodioloside)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Dopaminergic Neurons/*cytology/metabolism
MH  - Glucosides/*pharmacology
MH  - Mesenchymal Stem Cells/cytology/*drug effects
MH  - Microtubule-Associated Proteins/metabolism
MH  - Nerve Growth Factors/genetics/metabolism
MH  - Neurotrophin 3/genetics/metabolism
MH  - Phenols/*pharmacology
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Up-Regulation
PMC - PMC4410750
OTO - NOTNLM
OT  - dopaminergic neuron
OT  - mesenchymal stem cells
OT  - salidroside
EDAT- 2013/12/11 06:00
MHDA- 2014/10/31 06:00
PMCR- 2015/04/27
CRDT- 2013/12/11 06:00
PHST- 2013/07/18 00:00 [received]
PHST- 2013/11/13 00:00 [accepted]
PHST- 2013/12/11 06:00 [entrez]
PHST- 2013/12/11 06:00 [pubmed]
PHST- 2014/10/31 06:00 [medline]
PHST- 2015/04/27 00:00 [pmc-release]
AID - 10.1002/cbin.10217 [doi]
PST - ppublish
SO  - Cell Biol Int. 2014 Apr;38(4):462-71. doi: 10.1002/cbin.10217. Epub 2014 Jan 31.